Eupatorin was purchased from Extrasynthese (Genay Cedex, France). The following antibodies were used according to the manufacturer's instructions: poly(ADP-ribose) polymerase (PARP), mouse monoclonal; cytochrome c, mouse monoclonal; caspase-7, mouse monoclonal; caspase-8, rabbit polyclonal; Bax, rabbit polyclonal; Bid, rabbit polyclonal; and AIF, rabbit polyclonal (BD PharMingen, San Diego, CA, USA); Smac/DIABLO, mouse monoclonal (BD Transduction Laboratories); caspase-3, rabbit polyclonal (Assay Designs, Ann Arbor, MI, USA); caspase-4, caspase-6 and caspase-9, mouse monoclonal (Medical & Biological Laboratories, Nagoya, Japan); Bcl-2, mouse monoclonal (Santa Cruz Biotechnology, Santa Cruz, CA, USA); β-actin, mouse monoclonal (Sigma, Saint Louis, MO, USA); cytochrome c oxidase (Cox IV), mouse monoclonal (Abcam, Cambridge, UK); JNK/SAPK, Phospho-JNK/SAPK (phosphor T183 + Y185), p44/42 MAP Kinase, Phospho-p44/42 MAP Kinase (T202/Y204), p38MAPK and Phospho- p38MAPK (T180/Y182), rabbit polyclonal (New England BioLabs, Cell Signaling Technology, Beverly, MA, USA). Polyvinylidene-difluoride membranes were purchased from Millipore (Billerica, MA, USA). Secondary antibodies were from GE Healthcare Bio-Sciences AB (Little Chalfont, UK). All other chemicals were obtained from Sigma (Saint Louis, MO, USA).
P44 42 map kinase
Manufactured by Cell Signaling Technology
Sourced in United States
P44/42 MAP kinase is a lab equipment product that functions as an enzyme involved in the mitogen-activated protein kinase (MAPK) signaling pathway. It plays a crucial role in the regulation of cellular processes such as cell growth, proliferation, and differentiation.
Automatically generated - may contain errors
Lab products found in correlation
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Caspase 7, by BD (1 mentions)
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
Cytochrome c, by BD (1 mentions)
Caspase 8, by BD (1 mentions)
β actin, by Merck Group (1 mentions)
Bcl 2, by Santa Cruz Biotechnology (1 mentions)
Bcl2 associated x (bax), by BD (1 mentions)
Phospho p44 42 mapk, by Cell Signaling Technology (1 mentions)
Rsk1 rsk2 rsk3, by Cell Signaling Technology (1 mentions)
Phospho p90rsk, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Quetiapine, by AstraZeneca (1 mentions)
Phospho c raf ser259, by Cell Signaling Technology (1 mentions)
Nitrocellulose membrane, by Merck Group (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Protease and phosphatase inhibitor, by Roche (1 mentions)
Leupeptin, by Peptide Institute (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
5 protocols using p44 42 map kinase
1
Eupatorin-induced Apoptosis Signaling
2
Signaling Pathway Analysis of Antipsychotics
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All reagents were obtained from Sigma-Aldrich, Missouri, USA unless stipulated otherwise. Quetiapine was donated by AstraZeneca, Stockholm, Sweden; aripiprazole by Bristol-Myers Squibb, New Jersey, USA and AG1478 (EGFR inhibitor) purchased from A.G. Scientific, Inc., California, USA. Primary antibodies, including phospho-p44/42 MAPK, p44/42 MAP kinase, phospho-p90RSK, RSK1/RSK2/RSK3 and β-Actin were from Cell Signaling Technology, Massachusetts, USA and c-Fos from Assay Designs, Michigan, USA. Secondary antibodies, including goat anti-mouse and goat anti-rabbit horseradish peroxidase (HRP)-conjugated immunoglobulins (IgGs) were supplied by DAKO, NSW, Australia.
3
Western Blot Analysis of ERK1/2 and Ras Signaling
4
Immunodetection of Cellular Proteins
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Experiments for protein immunodetection were performed using the following antibodies: rabbit antibodies against EGFR (Cell Signaling Technology [4267] and Santa Cruz Biotechnology [sc-03]), GGA1 (Santa Cruz Biotechnology [sc-30102]), p44/42 MAP kinase (Cell Signaling Technology [4695]), p-p44/42 MAP kinase (Cell Signaling Technology [9101]), EEA1 (Cell Signaling Technology [2411]), CIMPR (Epitomics [5230-1]), and Alexa488-EEA1 (MBL [M176-A48]); mouse antibodies against EGFR (Millipore [05-101]), GAPDH (Santa Cruz Biotechnology [sc-32233]), β actin (Santa Cruz Biotechnology [sc-47778]), GGA2 (BD Transduction Laboratories [612612]), GGA3 (BD Transduction Laboratories [612310]), and GFP (Roche [11814460001]); a rat antibody against HA (Roche [11867423001]); a sheep antibody against TGN46 (Serotec [AHP500GT]). Immunohistofluorescence analyses of GGA2 were performed in paraffin-embedded tissues using an anti-GGA2 antibody that was generated by immunizing rabbits with a synthetic peptide carrying the sequence QNPSADRNLL, which lies within the hinge domain of human GGA2. The specificities of this antibody were demonstrated in Supplementary Fig. S5 .
Cetuximab (Erbitux) were purchased from MERCK. E64d and Leupeptin were purchased from Peptide Institute, Inc.
Cetuximab (Erbitux) were purchased from MERCK. E64d and Leupeptin were purchased from Peptide Institute, Inc.
5
Immunoblotting Analysis of Cell Signaling
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Cells were treated with vehicle or NOB for the indicated periods. Total protein lysates were extracted using RIPA buffer (Thermo Fisher Scientific), and the protein concentration was measured by staining with Bradford dye reagent (Bio-Rad). Equal amounts of protein were separated by 10% SDS-PAGE, followed by transfer to polyvinylidene difluoride (PVDF) membranes. Membranes were incubated with the following antibodies: integrin subunit beta 3 (CD61), integrin subunit alpha 2b (CD41), p21 Waf1/Cip1 (p21), cyclin D2, cyclin E1, cyclin-dependent kinase 6 (CDK6), phospho-p44/42 MAP Kinase, p44/42 MAP Kinase, EGR1 (Cell Signaling Technology, Danvers, MA, USA), p27 Kip1 (p27) (ABclonal, Woburn, MA, USA), and actin (Thermo Fisher Scientific). The blots were then incubated with the appropriate horse-radish peroxidase (HRP)-conjugated secondary antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Proteins were detected using Amersham ECL Prime Western Blotting Detection Reagent, and the signal was visualized on Amersham Hyperfilm ECL (GE Healthcare).
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