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2 protocols using gd no3 3.6h2o

1

Multifunctional PAMAM Dendrimer Synthesis

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Ethylenediamine core G5.NH2 PAMAM dendrimers (molecular weight = 26,010 g/mol) with polydispersity index values less than 1.08 were purchased from Dendritech (Midland, MI, USA). Polyethylene glycol (PEG) monomethyl ether with a carboxyl group at one end (mPEG-COOH) was obtained from Shanghai Yanyi Biotechnology Corporation (Shanghai, China). Gd(NO3)3.6H2O, MnSO4.H2O, HAuCl4.4H2O, acetic anhydride, triethylamine, and all other chemicals as well as solvents were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Sodium borohydride was purchased from J&K Chemical Ltd. (Shanghai, China). DOTA-NHS was purchased from CheMatech (Dijon, France). The water used in all experiments was purified using a Milli-Q Plus185 water purification system (Millipore, Bedford, MA) with a resistivity greater than 18.2 MΩ cm.
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2

Gd(III)-Based Theranostic Nanoprobe

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Bovine serum albumin (BSA) was obtained from Solarbio (Beijing, People’s Republic of China). Gd(NO3)3·6H2O, hexadecyl trimethyl ammonium bromide (CTAB), and dimethyl sulfoxide (DMSO) were purchased from Sinopharm Chemical Reagent Co. Ltd., Beijing, People’s Republic of China Tetraeth-ylorthosilicate (TEOS), poly(dimethyl diallyl ammonium chloride) (PDDA), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl), and N-hydroxysuccinimide (NHS) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). DNA oligos were synthesized and purified by Shanghai Sangon Biotechnology Co. Ltd. (Shanghai, People’s Republic of China); 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Sunshine Biotech. Co. Ltd. (Nanjing, People’s Republic of China). Cell culture media including HDMEM, RPMI 1640, and fetal calf serum were obtained from Kangmei Biotechnology Co. Ltd (Xuzhou, People’s Republic of China). All other reagents were of analytical grade and used without purification. All aqueous solutions were prepared using ultrapure water from a Milli-Q system (Millipore, Boston, MA, USA). The DNA sequence is listed as follows.
COOH-AS1411:
5′-COOH-GGTGGTGGTGGTTGTGGTGGTGGTGG-3′.
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