Anti otub1

Manufactured by Cell Signaling Technology

Anti-OTUB1 is a laboratory reagent produced by Cell Signaling Technology. It is an antibody that recognizes the OTUB1 protein. OTUB1 is a deubiquitinating enzyme involved in the regulation of cellular processes. The primary function of Anti-OTUB1 is to detect and quantify OTUB1 expression in biological samples.

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Lab products found in correlation

2 protocols using anti otub1

HIF-1α Regulation by Ubiquitin Pathway

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Antibodies including anti-OTUB1 (#3783), anti-HIF-1α (#36169), anti-VHL (#68547), anti-FIH (#4426), anti-Ubiquitin (#3936), anti-K48-linkage Specific Polyubiquitin (#8081), and normal rabbit IgG (#2729) were purchased from Cell Signaling Technology. Anti-ACTB (#AC026) antibody was purchased from ABclonal. Anti-Flag (#F1804) antibody was purchased from Sigma. Anti-HA (#901515) antibody was purchased from Covance. Anti-Myc (#SC-40) antibody was purchased from Santa Cruz Biotechnology. CoCl₂ (#C8661), Deferoxamine mesylate salt (DFX) (#D9533), DMOG (#D3695) and MG-132 (#474790) were purchased from Sigma. FG4592 (#S1007) was purchased from Selleck. The cells were treated with DMOG (1 mM) or FG4592 (up to 100 μM) for 6–8 h, and DMSO was used as a control.

Liu X., Deng H., Tang J., Wang Z., Zhu C., Cai X., Rong F., Chen X., Sun X., Jia S., Ouyang G., Li W, & Xiao W. (2022). OTUB1 augments hypoxia signaling via its non-canonical ubiquitination inhibition of HIF-1α during hypoxia adaptation. Cell Death & Disease, 13(6), 560.

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Antibody Reagents for Protein Analysis

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Antibodies including anti-OTUB1 (#3783), anti-SET7 (#2825), anti-His (#9991), anti-Histone H3 (#4499), anti-UBC13 (#6999), anti-HA (#3724), anti-Ubiquitin (#3936), and normal rabbit IgG (#2729) were purchased from Cell Signaling Technology. anti-OTUB1 (#ab270959) and anti-SET7 (#ab124708) antibodies were purchased from Abcam. Anti-β-actin (#AC026) antibody was purchased from ABclonal. Anti-Flag (#F1804) antibody was purchased from Sigma. anti-HA (#901515) antibody was purchased from Covance. Anti-Myc (#SC-40) antibody was purchased from Santa Cruz Biotechnology. Anti-α-tubulin (#62204) and Alexa Fluor 488 goat anti-rabbit IgG (#A11008) were purchased from Thermo Fisher Scientific. Erastin (#HY-15763) was purchased from MCE. TBH solution (#B802372) was purchased from Macklin. CHX (#HY-12320) was purchased from MCE.

Deng H., Jia S., Tang J., Rong F., Xu C., Chen X., Wang Z., Zhu C., Sun X., Liao Q., Liu W., Li W., Xiao W, & Liu X. (2023). SET7 methylates the deubiquitinase OTUB1 at Lys 122 to impair its binding to E2 enzyme UBC13 and relieve its suppressive role on ferroptosis. The Journal of Biological Chemistry, 299(4), 103054.

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