Bone sections were stained for TRAP using a TRAP & ALP double-stain Kit (Takara Bio, Mountain View, CA) and analyzed for osteoclastic bone destruction using Magnafire 4.1 software (Optronics, Goshen, IN) under a microscope (IX81, Olympus). TRAP+OCs were counted at tumor-bone interface at endocortical bone using three levels of sections for each sample according to the methods previously reported [24] (link). Data are expressed as the number of osteoclasts/bone surface (mm) (No. OC/BS/mm).
Magnafire 4
Manufactured by Optronics
Magnafire 4.1 is a software application developed by Optronics for use with their range of lab equipment. The software's core function is to provide a user interface for controlling and monitoring the operation of Optronics' laboratory instruments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using magnafire 4
1
TRAP-Stained Osteoclast Quantification
2
Histological Analysis of Bone TRAP Activity
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