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Trichloroacetic acid precipitation kit

Manufactured by Abcam
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The Trichloroacetic acid (TCA) precipitation kit is a laboratory tool used to separate proteins from other components in a sample. It utilizes the precipitating properties of TCA to isolate proteins, which can then be further analyzed or processed.

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Lab products found in correlation

Citrate assay kit, by Abcam (2 mentions) Alanine transaminase activity assay kit, by Abcam (2 mentions)

2 protocols using trichloroacetic acid precipitation kit

1

Liver Function and Citrate Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Liver function and damage was determined by measuring alanine transaminase (ALT). The ALT activity in the plasma was determined using the Alanine Transaminase Activity Assay Kit (Abcam ab105134) following manufacturer’s protocol. The plasma ALT levels over two different time points were obtained and corresponding ALT activity was determined by reading in a plate reader at 570 nm. Plasma and extracellular citrate levels were determined using the Citrate Assay Kit (Abcam ab83396) by reading in a plate reader at 570 nm following the manufacturer’s protocol. When required, deproteination was accomplished using a trichloroacetic acid precipitation kit (Abcam ab204708) for tissue and cells, or 10,000 MWCO spin column for plasma.
Madaris T.R., Venkatesan M., Maity S., Stein M.C., Vishnu N., Venkateswaran M.K., Davis J.G., Ramachandran K., Uthayabalan S., Allen C., Osidele A., Stanley K., Bigham N.P., Bakewell T.M., Narkunan M., Le A., Karanam V., Li K., Mhapankar A., Norton L., Ross J., Aslam M.I., Reeves W.B., Singh B.B., Caplan J., Wilson J.J., Stathopulos P.B., Baur J.A, & Madesh M. (2023). Limiting Mrs2-dependent mitochondrial Mg2+ uptake induces metabolic programming in prolonged dietary stress. Cell reports, 42(3), 112155.
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2

Liver Function and Citrate Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Liver function and damage was determined by measuring alanine transaminase (ALT). The ALT activity in the plasma was determined using the Alanine Transaminase Activity Assay Kit (Abcam ab105134) following manufacturer’s protocol. The plasma ALT levels over two different time points were obtained and corresponding ALT activity was determined by reading in a plate reader at 570 nm. Plasma and extracellular citrate levels were determined using the Citrate Assay Kit (Abcam ab83396) by reading in a plate reader at 570 nm following the manufacturer’s protocol. When required, deproteination was accomplished using a trichloroacetic acid precipitation kit (Abcam ab204708) for tissue and cells, or 10,000 MWCO spin column for plasma.
Stowe-Evans E.L., Harper R.M., Motchoulski A.V, & Liscum E. (1998). NPH4, a Conditional Modulator of Auxin-Dependent Differential Growth Responses in Arabidopsis. Plant Physiology, 118(4), 1265-1275.
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