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2 protocols using isorhoifolin

1

HPLC Analysis of Flavonoid Compounds

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HPLC-grade acetonitrile and methanol, as well as vicenin-2, vitexin, isovitexin, roipholin, isorhoifolin, and apigenin, were supplied by Sigma-Aldrich (St. Louis, MO, USA), while dimethylformamide (DMF) was supplied by Carlo Erba (Milano, Italy). All the other reagents and chemicals used in this study were of analytical grade and were purchased from Sigma (Sigma-Aldrich GmbH, Sternheim, Germany).
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2

HPLC Analysis of Phenolic Compounds

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Reagents for HPLC analysis of phenolic compounds, methanol, and acetonitrile (HPLC grade) were purchased from SDS (Peypin, France), phosphoric acid from Probus (Badalona, Spain) and acetic acid from Merck (Darmstadt, Germany). Pure water was obtained from Milli-Q (Millipore, MA, USA). Phenolic standards: protocatechuic acid, coumaric acid, quercetin, β-hydroxyl benzoic acid, alloevodionol, 5-hydroxyverotric acid, chlorogenic acid, neptein,3,4,'-dimethoxychrysin, 3,4'dimethoxychrysin, epicatechin, catechin, gallic acid, ferulic acid, caffeic acid, chlorogenic acid, trimethoxy quercetin, apigenin hyperoside, kaempferol, kaempferol-3-O-glucoside, keampferol-3rutinoside, kaempferol-7-O-neohesperidoside, quercitrin, isoquercitrin, iso-rhamnetin, luteolin, luteolin-6-C-glucoside, luteolin-8-C-glucoside, luteolin-7-O-glucoside, apigenin, apigenin-7-O-glucoside, apigenin 6-arabinose, apigenin-8-C-glucoside, amentoflavone, naringin, naringenin, naringenin-7-O-glucoside, isorhoifolin and rutin were purchased from Sigma Co. (St. Louis, MO, USA). The purity of the standards was 98%.
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