DLBCL cell lines (0.25 × 106 cells/ml) were exposed in vitro to pevonedistat (0.313 to 10 µM) for 24–72 h at 37°C and 5% CO2. Cell viability changes were determined by measuring adenosine triphosphate (ATP) content using the Cell Titer‐Glo Luminescent Viability Assay (Promega, Madison, WI). The half‐maximal inhibitory concentration (IC50) of pevonedistat was calculated using the Graph Pad Prism Software Version 6.04 (Graph Pad Software, Inc., La Jolla, CA). Primary tumor cells isolated from B‐cell lymphoma patients (1 × 106 cells/mL) were exposed to pevonedistat (0.5 µM) for primary lymphoma samples or vehicle control (Dimethyl sulfoxide [DMSO] 0.001%) for 24, 48 and 72 h.
Graph pad prism software version 6
Manufactured by GraphPad
Sourced in United States
GraphPad Prism Software Version 6.04 is a data analysis and graphing software designed for scientific research. The software provides tools for data management, statistical analysis, and visualization of scientific data.
Automatically generated - may contain errors
4 protocols using graph pad prism software version 6
1
Evaluating Pevonedistat Cytotoxicity in DLBCL
2
Statistical Analyses of Biological Data
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Biostatistical analyses were performed by the GraphPadPrism® Software Version 6.04 (GraphPad Software, Inc., San Diego, U.S.A.). The D′Agostino-Pearson omnibus test was used to test for normal distribution, and comparisons of normally-distributed values were achieved with an unpaired t-test or one-way ANOVA. Pearson´s correlation coefficients were calculated to analyze associations between two parameters. IBM® SPSS® Statistics, Version 22 (IBM Corp., Armonk software) was used for linear regression analyses. The significance of the results was expressed as the P-value (two-tailed) which is interpreted as followed: P<0.0001 # and P<0.001*** are extremely significant, P<0.01** is very significant, P<0.05* is significant and P≥0.05 is not significant. The software Table Curve® 2D Version 5.01 (Systat Software GmbH, Erkrath, Germany) was used for the precision profile (Fig. 4 ), and for the non-linear curve fitting in the stability results (Fig. 5 ).
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Evaluating Pevonedistat Cytotoxicity in DLBCL
4
Evaluating AMG176 Cytotoxicity in DLBCL
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DLBCL, DHL and BL cell lines (0.25×10 6 cells/ml) were exposed in vitro to AMG176 (0.039 to 20µM) for 24-72 hours at 37°C and 5% CO 2 . Cell viability changes were determined by measuring adenosine triphosphate (ATP) content using the Cell Titer-Glo Luminescent Viability Assay (Promega, Madison, WI). The half-maximal inhibitory concentration (IC 50 ) of AMG176 was calculated using the Graph Pad Prism Software Version 6.04 (Graph Pad Software, Inc., La Jolla, CA).
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