Actin egfp

C57BL/6 (WT) mice were obtained from Charles River Laboratories. CCR2-RFP, Actin-eGFP, IL12p40-eYFP, Il12b^−/−, ifngr1^−/−, LysM^cre, Csf1r^LsL-DTR, Ccr7^−/−, OTII (male-linked) and Thy1.1 mice were obtained from Jackson Laboratories. Ifnar^−/− mice (Müller et al., 1994 (link)), were a gift from Michel Aguet (University of Zurich, Zurich, Switzerland). REX3-Tg mice were generated by our lab as previously described (Groom et al., 2012 (link)). Kaede transgenic mice were obtained from Dr. Osami Kanagawa (RIKEN Institute) (Tomura et al., 2008 (link)), rederived at Taconic and then bred at Massachusetts General Hospital. LysM^cre mice were crossed with Csf1r^LsL-DTR to generate MM-DTR mice as previously described (Schreiber et al., 2013 (link)). All mice were maintained on the C57BL/6 background. REX3-Tg and IL12p40-eYFP mice were bred and maintained in specific pathogen free (SPF) plus Helicobacter and Pasteurella Pneumotropica free conditions at the Massachusetts General Hospital all others were bred and maintained under SPF conditions. Male and female mice were used at 6-12 weeks. All procedures were approved and carried out according to the standards and guidelines set forth by the Institutional Animal Care and Use Committee of Massachusetts General Hospital.