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Xlumplanfl 20 0.95 na water immersion

Manufactured by Olympus
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The XLUMPlanFL 20× 0.95 NA water-immersion objective is a high-numerical aperture (NA) lens designed for water-immersion microscopy applications. It provides a 20× magnification and a numerical aperture of 0.95, enabling high-resolution imaging and light collection.

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Lab products found in correlation

Bp 405, by Thorlabs (2 mentions) Igor software, by Wavemetrics (2 mentions)

2 protocols using xlumplanfl 20 0.95 na water immersion

1

Light Stimulation Characterization of Retinal Responses

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Light stimulation was generated by custom-written code in Igor software (Wavemetrics) and 4D Workshop 4 IDE (4D Systems) to control an LCD mask in front of a collimated LED (405 nm, Thorlabs) with a bandpass filter (BP 405, Thorlabs). The stimuli were projected onto the retina through the objective lens (XLUMPlanFL 20× 0.95 NA water-immersion, Olympus). Stimulus contrast varied between 100 – 300%, with the 300% stimulus intensity at ~25 × 103 photons s−1 μm−2 on a background intensity of ~6 × 103 photons s−1 μm−2. For the bar stimulus, the bar (400 × 400 μm) moved in one of eight evenly – spaced directions at a range of velocities between 0.03 – 1 mm s−1. The bullseye stimulus was configured as previously described6 (link). Each stimulus was repeated 3 – 5 times and responses were averaged.
Ding H., Smith R.G., Poleg-Polsky A., Diamond J.S, & Briggman K.L. (2016). Species-specific wiring for direction selectivity in the mammalian retina. Nature, 535(7610), 105-110.
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2

Light Stimulation Characterization of Retinal Responses

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Light stimulation was generated by custom-written code in Igor software (Wavemetrics) and 4D Workshop 4 IDE (4D Systems) to control an LCD mask in front of a collimated LED (405 nm, Thorlabs) with a bandpass filter (BP 405, Thorlabs). The stimuli were projected onto the retina through the objective lens (XLUMPlanFL 20× 0.95 NA water-immersion, Olympus). Stimulus contrast varied between 100 – 300%, with the 300% stimulus intensity at ~25 × 103 photons s−1 μm−2 on a background intensity of ~6 × 103 photons s−1 μm−2. For the bar stimulus, the bar (400 × 400 μm) moved in one of eight evenly – spaced directions at a range of velocities between 0.03 – 1 mm s−1. The bullseye stimulus was configured as previously described6 (link). Each stimulus was repeated 3 – 5 times and responses were averaged.
Ding H., Smith R.G., Poleg-Polsky A., Diamond J.S, & Briggman K.L. (2016). Species-specific wiring for direction selectivity in the mammalian retina. Nature, 535(7610), 105-110.
+ Open protocol
+ Expand

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