The paraformaldehyde-fixed gastric tissues were embedded in paraffin and cut into sections, dewaxed and hydrated. After blocking the appropriate antisera, the sections were incubated with CDX2 (1:100 dilution; Abcam, ab157524), MUC2 (1:100 dilution; Abcam, ab76774), ki-67 (1:100 dilution; Abcam, ab16667), p53 (1:100 dilution; Millipore, #CBL404), and PTEN (1:100 dilution; Abcam, ab238032). Then, the sections were incubated with anti-rabbit or anti-mouse IgG (Boshide Biological Technology, Wuhan, China). Positive signals were visualized using a diaminobenzidine kit (GTVisionTM III Detection System/Mo&Rb; Gene Group Holdings, Shanghai, China), and the sections were counterstained with haematoxylin. Image-Pro was used to analyse the integrated optical density (IOD) and area of each picture. The mean density (IOD/area) was used to analyse the protein expression.
Ab157524
Manufactured by Abcam
Ab157524 is a laboratory reagent produced by Abcam. It is a recombinant antibody fragment that can be used in various immunoassay and cell-based applications. The product details and intended use are not provided to maintain an unbiased and factual description.
Automatically generated - may contain errors
Lab products found in correlation
Ab16667, by Merck Group (1 mentions)
Ab76774, by Abcam (1 mentions)
Alexa fluor 488 conjugated anti rabbit, by Thermo Fisher Scientific (1 mentions)
Ab5603, by Merck Group (1 mentions)
Ab6046, by Abcam (1 mentions)
Ab76541, by Abcam (1 mentions)
Hfab rhodamine anti tubulin primary antibody, by Bio-Rad (1 mentions)
Alexafluor 647 conjugated anti mouse, by Thermo Fisher Scientific (1 mentions)
2 protocols using ab157524
1
Immunohistochemical Analysis of Gastric Tissues
2
Embryonic Protein Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies used for analysis of embryos include rabbit anti–β-tubulin (Abcam, ab6046, polyclonal, lot: GR31927-5), mouse anti–DICER-1 (Santa Cruz Biotechnology, sc-136981, A-2, lot: I1817), mouse anti–CDX-2 (Abcam, ab157524, CDX2-88, lot: GR300552-6), anti-rabbit anti-CDX2 antibody (Abcam, ab76541, lot #GR300552-18), rabbit anti-SOX-2 (EMD Millipore, AB5603, polyclonal, lot: NG1863962 and 3254559), goat anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich, SAB2500450, polyclonal, lot: 6377C2), rabbit anti-GADD45a (Invitrogen, MA5-17014, D.81.E, lot: R12274975), Alexa Fluor 647 anti-mouse/human CD324 (E-cadherin) antibody (BioLegend catalog no. 147307, lot no. B254794), Alexa Fluor 594 FGFR-1 antibody (Santa Cruz Biotechnology, catalog no. sc-57132 AF594, lot no. J0218), Rhodamine anti–β-actin immunoglobulin G (Bio-Rad, 12004163, lot: 64219909), hFAB Rhodamine anti-tubulin primary antibody (Bio-Rad, 12004165, lot no. 64210670). Donkey secondary antibodies Alexa Fluor 647–conjugated anti-mouse (A31571, lot #1903516), Alexa Fluor 594–conjugated anti-mouse (A21203, lot #2066086), and Alexa Fluor 488-conjugated anti-rabbit (A21206) were purchased from Thermo Fisher Scientific, CA, USA. All bands migrated to approximate molecular masses stated by manufacturers or previously published studies (table S2).
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