Alexa fluor 488 conjugated anti mouse immunoglobulin g igg

Dulbecco’s modified Eagle medium (DMEM), Alexa Fluor 488-conjugated anti-mouse immunoglobulin G (IgG), and 4′,6 diamidino-2-phenylindole dihydrochloride (DAPI) were obtained from Thermo Scientific (Rockford, IL, USA). Antibiotic-antimycotic reagent and fetal bovine serum (FBS) were purchased from Gibco BRL (Grand Island, NY, USA). TAK242, LPS (Escherichia coli, serotype 011:B4), 3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), anti-β-actin antibody, and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Antibodies specifically recognizing TLR4, phosphorylated IκBα, and lamin B1 were supplied by Abcam (Cambridge, MA, USA), and antibodies recognizing iNOS, COX2, MyD88, NF-κB p65, non-phospho-IκBα, and horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse IgG were purchased from Cell Signaling Technology (Danvers, MA, USA).

Aze was purchased from Selleckchem (Houston, TX, USA). SP600125 was obtained from Calbiochem (Darmstadt, Germany). LPS (Escherichia coli, serotype 011:B4), mouse anti-β-actin (1:2000, cat# A5316) and rabbit anti-lamin B1 (1:2000, cat# SAB1306342) antibodies, dimethyl sulfoxide (DMSO), and adenylyl-imidodiphosphate (AMPPNP) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Alexa Fluor 488-conjugated anti-mouse immunoglobulin G (IgG) and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) were obtained from Thermo Scientific (Rockford, IL, USA). Antibiotic-antimycotic reagent, Dulbecco’s modified Eagle medium (DMEM), and fetal bovine serum (FBS) were purchased from Gibco BRL (Grand Island, NY, USA). The rabbit anti-phospho-IκBα (1:1000, cat# ab97783) antibody was supplied by Abcam (Cambridge, MA, USA). Rabbit anti-phospho-JNK (1:1000, cat# 4668), rabbit anti-JNK3 (1:1000, cat# 2305), rabbit anti-phospho-c-Jun (1:1000, cat# 9261), rabbit anti-c-Jun (1:1000, cat# 9165), rabbit anti-iNOS (1:1000, cat# 2982), rabbit anti-COX2 (1:1000, cat# 4842), mouse anti-NF-κB p65 (1:1000, cat# 6956), mouse anti-IκBα (1:1000, cat# 4814) antibodies, and horseradish peroxidase-conjugated anti-rabbit (1:2000, cat# 7074) and anti-mouse IgG (1:2000, cat# 7076) were purchased from Cell Signaling Technology (Danvers, MA, USA).