All DNA transfections were carried out using Lipofectamine 3000 (Thermo Fisher) according to the manufacturer’s instructions. All transcriptional effector experiments were carried out at a mass ratio between 1:2 and 1:3 of Gal4DB constructs to the transcriptional effector. For imaging experiments, fibronectin (Product #F1141, Sigma-Aldrich) was seeded on to glass coverslip substrate at a dilution of 5 μg/mL for 1 h at RT and rinsed three times with PBS before seeding cells. All other experiments were carried out on tissue cultured treated plastic (Corning) or for cell adherence by the manufacturer (ibiTreat by Ibidi).
The drug was either added at the time of transfection or 24 h later. Grazoprevir, danoprevir, telaprevir, and glecaprevir were purchased from MedChemExpress. Stocks were prepared at between 3 −10 mM concentrations between 3 and 10 mM by dissolution in DMSO.
The drug was either added at the time of transfection or 24 h later. Grazoprevir, danoprevir, telaprevir, and glecaprevir were purchased from MedChemExpress. Stocks were prepared at between 3 −10 mM concentrations between 3 and 10 mM by dissolution in DMSO.