The Superscript III One‐Step RT‐PCR Kit with Platinum Taq polymerase is a reagent kit designed for reverse transcription and polymerase chain reaction (RT‐PCR) in a single-tube format. It contains the necessary components for both reverse transcription and subsequent PCR amplification.
30 minutes 48°C, 2 minutes 94°C, 45 cycles each of 30 seconds 94°C ‐ 30 seconds 55°C ‐ 40 seconds 68°C, final elongation 2 minutes 68°C.
30 minutes 50°C, 2 minutes 94°C, 45 cycles each of 30 seconds 94°C – 45 seconds 50°C – 45 seconds 68°C, final elongation 5 minutes 68°C.
30 minutes 48°C, 2 minutes 94°C, 45 cycles each of 30 seconds 94°C – 30 seconds 55°C – 40 seconds 68°C, final elongation 2 minutes 68°C.
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