Infinity 2 system

NMR data (¹H, ¹³C{¹H}) were recorded on a 400 MHz Varian Unity Inova spectrometer. IR spectroscopy was performed using a Thermo Nicolet Avatar 330 FT-IR with ATR crystal. Mass spectrometry was performed on a Waters Synapt G2 with an ESI probe in ESI positive mode using a Cone Voltage of 15 V. HPLC data were recorded on an Agilent infinity II system. Flash chromatography was done using a Biotage Isolera One. Cyclic voltammograms were recorded using a NuVant System EZstatpro. UV-vis was performed using the Agilent Cary 3500 UV-Vis Spectrophotometer. Healthy human cell lines HEK293 and PNT1A were supplied by Dr CH Kaschula (University of Stellenbosch). Growth of HEK293 and PNT1A cells was monitored using a BioTek Synergy HTX multi-mode plate reader. T. gondii ΔKu80:mNeon cells used were provided by Dr CR Harding. Growth inhibition assays of T. gondii were monitored using a BMG LabTech PHERAstar FS microplate reader. Fluorescence activated cell sorting was performed using a Celesta FACS instrument.

The purity of catalase was examined by a size-exclusion column with a separation range of 10,000 Da–10,00,000 Da BioCore SEC-300) using a high-performance liquid chromatography (HPLC, 1,260 Infinity II system, Agilent) system. Transmission electron microscope (TEM, HT7700, Hitachi Ltd.) was employed to characterize the morphologies of CAT-PEG. Dynamic light scattering (DLS) measurements were performed on a Zetasizer Nano instrument (Malvern Instruments Ltd., United Kingdom) with a 10–mW helium–neon laser and thermoelectric temperature controller. Fluorescent intensity was measured with a Spectra Max M2 plate reader (Molecular Devices). The bioluminescent imaging of the mice was imaged with the IVIS Imaging System (IVIS Spectrum, PerkinElmer). H and E images were taken using inverted fluorescence microscope fluorescence microscope (BK-FL4, ChongQing Optec Instrument Co., Ltd.). TUNEL images were taken using a confocal laser scanning microscopy (Leica TCS SP8). The complete blood count and blood biochemical examination were performed using auto biochemistry analyzer (BS-420, Mindray) and auto hematology analyzer (RJ-0C107223, Mindray), respectively.