Trichloroacetic acid tca

Manufactured by Junsei

Sourced in Japan

Trichloroacetic acid (TCA) is a colorless crystalline solid that is used as a laboratory reagent. It has the chemical formula CCl3COOH and a molecular weight of 163.39 g/mol. TCA is commonly used for protein precipitation, deproteinization, and as a fixative in histology and biochemistry applications.

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Lab products found in correlation

2 protocols using trichloroacetic acid tca

Antioxidant Capacity of Tomato Cultivars

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Fully ripened tomatoes (Lycopersicon esculentum Mill) were purchased from a wholesale market in Gwangju, Korea. Folin-Ciocalteu reagent, linoleic acid, ethylene diaminetetra acetic acid (EDTA), 1,1-diphenyl-2-pycrylhydrazyl (DPPH)-radical, and 2-thiobarbituric acid (TBA) were purchased from Sigma-Aldrich (Germany). Ferrous chloride, gallic acid, ferric chloride, petroleum ether, ascorbic acid, and trichloroacetic acid (TCA) were obtained from Junsei Chemical (Japan). Potassium ferricyanide was obtained from Avocado Research Chemicals (UK). Plate count agar and violet red bile agar were purchased from Difco (USA).

Kim H.S, & Chin K.B. (2016). Effects of Drying Temperature on Antioxidant Activities of Tomato Powder and Storage Stability of Pork Patties. Korean Journal for Food Science of Animal Resources, 36(1), 51-60.

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Antioxidant and Anti-inflammatory Assays

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Lipopolysaccharide (LPS, Escherichia coli O127:B8), 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH), 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), dimethyl sulfoxide (DMSO), Folin–Ciocalteu′s phenol reagent, 2,4,6-tris(2-pyridyl)-1,3,5-triazine (TPTZ), gallic acid, catechin, sodium nitroprusside, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA), and Trolox were purchased from Sigma-Aldrich (St. Louis, MO, USA). Trichloroacetic acid (TCA) and 12-molybdo(VI) phosphoric acid b-hydrate were obtained from Junsei Chemical Co. (Tokyo, Japan). Anti-COX-2, iNOS, and HRP-conjugated anti-rabbit secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-IL-1β, CAT, CAT, SOD-2, and HRP-conjugated anti-mouse secondary antibodies were acquired from Santa Cruz Biotechnology (Dallas, TX, USA). All other reagents are of analytical grade.

Rarison R.H., Truong V.L., Yoon B.H., Park J.W, & Jeong W.S. (2023). Antioxidant and Anti-Inflammatory Mechanisms of Lipophilic Fractions from Polyscias fruticosa Leaves Based on Network Pharmacology, In Silico, and In Vitro Approaches. Foods, 12(19), 3643.

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