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Model 1100 b

Manufactured by PerkinElmer
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The Model 1100 B is a versatile lab equipment designed for various analytical applications. It features advanced technology and precise measurements to support researchers and scientists in their work. The core function of this model is to provide reliable and accurate data to support scientific discoveries and insights.

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Model 1100 (6 citations)

2 protocols using model 1100 b

1

Quantifying CO Dehydrogenase Cofactors

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Sulfane sulfur was determined by treating samples of CO dehydrogenase with potassium cyanide followed by colorimetric analysis of the resulting thiocyanate as FeSCN [12 (link)]. FAD was determined from its absorption at 450 nm in supernatants of trichloroacetic acid precipitates of CO dehydrogenase, neutralized with 2.4 mM K2HPO4 [9 (link)]. Copper, molybdenum and zinc in CO dehydrogenase were determined by flame atomic absorption spectroscopy (model 1100 B, Perkin Elmer, Überlingen, Germany).
Pelzmann A.M., Mickoleit F, & Meyer O. (2014). Insights into the posttranslational assembly of the Mo-, S- and Cu-containing cluster in the active site of CO dehydrogenase of Oligotropha carboxidovorans. Journal of Biological Inorganic Chemistry, 19(8), 1399-1414.
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2

Cultivation of B. japonicum USDA110

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B. japonicum USDA110 is the parent strain used in this study. B. japonicum strains were routinely grown at 29°C in GSY medium as described previously [20 (link)]. The actual iron concentration of the unsupplemented medium was 0.3 μM, as determined with a PerkinElmer Life Sciences model 1100B atomic absorption spectrometer.
Sankari S, & O’Brian M.R. (2016). Synthetic Lethality of the bfr and mbfA Genes Reveals a Functional Relationship between Iron Storage and Iron Export in Managing Stress Responses in Bradyrhizobium japonicum. PLoS ONE, 11(6), e0157250.
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