All media components were purchased from ThermoFisher or VWR. All enzymes were purchased from New England Biolabs unless otherwise specified. The recombinant SARS-CoV-2 Spike S1-hFc-His tagged protein used for titrations and sorting was purchased from ThermoFisher (RP-876–79). The recombinant neuraminidase (NA) for titrations was obtained through BEI Resources, NIAID, NIH: N2 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Brisbane/10/2007 (H3N2), Recombinant from Baculovirus, NR-43784. The ectodomain of A/Brisbane/02/2018 H1 HA with a foldon trimerization domain was expressed in HEK293T cells (ATCC) and purified using Ni-NTA affinity chromatography. Recombinant neuraminidase and recombinant hemagglutinin were biotinylated in a 20:1 molar ratio of biotin to antigen with EZ-Link NHS-Biotin (ThermoFisher, 20217) following the manufacturer’s instructions.
Recombinant sars cov 2 spike s1 hfc his tagged protein
Manufactured by Thermo Fisher Scientific
Recombinant SARS-CoV-2 Spike S1-hFc-His tagged protein is a laboratory reagent. It is a recombinant protein consisting of the S1 subunit of the SARS-CoV-2 spike protein fused to a human Fc domain and a histidine tag. This protein can be used for research purposes.
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Lab products found in correlation
2 protocols using recombinant sars cov 2 spike s1 hfc his tagged protein
1
SARS-CoV-2 Spike and Influenza Protein Production
2
Recombinant Protein Reagent Preparation
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All media components were purchased from ThermoFisher or VWR. All enzymes were purchased from New England Biolabs unless otherwise specified. The recombinant SARS-CoV-2 Spike S1-hFc-His tagged protein used for titrations and sorting was purchased from ThermoFisher (RP-876-79). The recombinant neuraminidase (NA) for titrations was obtained through BEI Resources, NIAID, NIH: N2 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Brisbane/10/2007 (H3N2), Recombinant from Baculovirus, NR-43784. The ectodomain of A/Brisbane/02/2018 H1 HA with a foldon trimerization domain was expressed in HEK293T cells (ATCC, female, #CRL-11268) and purified using Ni-NTA affinity chromatography. Recombinant neuraminidase and recombinant hemagglutinin were biotinylated in a 20:1 molar ratio of biotin to antigen with EZ-Link NHS-Biotin (ThermoFisher, 20217) following the manufacturer’s instructions.
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