Plasma was prepared from whole blood after centrifugation (10 min, 1300×g followed by 15 min, 2400×g) and transferring supernatant. Samples were stored at − 80 °C until analysis. Plasma levels of ALT were assessed using the mouse ALT ELISA Kit (Abcam, ab282882) according to the manufacturer’s instructions.
Mouse alt elisa kit
Manufactured by Abcam
Sourced in United States
The Mouse ALT ELISA Kit is a quantitative assay designed to measure the levels of alanine aminotransferase (ALT) in mouse serum or plasma samples. ALT is an enzyme found in the liver and its detection can be used as a biomarker for liver health.
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Lab products found in correlation
Mouse ast elisa kit, by Abcam (2 mentions)
Legendplex mouse inflammation panel, by BioLegend (1 mentions)
Mouse rat angiopoietin 2 quantikine elisa kit, by R&D Systems (1 mentions)
Kx 21n haematology analyser, by Sysmex (1 mentions)
Cobas total cholesterol 106570, by Roche (1 mentions)
Microplate reader, by Bio-Rad (1 mentions)
Mouse tnf α elisa kit, by Abcam (1 mentions)
Mouse il 6 elisa kit, by Abcam (1 mentions)
Simplestep elisa kit, by Abcam (1 mentions)
7 protocols using mouse alt elisa kit
1
Plasma Preparation and ALT Assay
2
Comprehensive Blood and Cytokine Analysis
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RBC count, haemoglobin, and mean cell volume was measured from whole blood using an automatic KX-21N Haematology Analyser (Sysmex). Serum levels of ANG-2 and ALT were measured according to the producers’ instructions, using the Mouse ALT ELISA Kit (ab282882, Abcam) and the Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit (MANG20, R&D Systems), respectively. Serum cytokines were measured using the LEGENDplex Mouse Inflammation Panel (740446, BioLegend) bead-based immunoassay. The assay was performed according to the manufacturer’s instructions, except that the protocol was adapted to use half-volumes.
3
Lipid Metabolism Biomarker Monitoring
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Every 4 weeks, after 4-h fasting (9:00-13:00), tail vein blood (n = 16 per group) was collected into paraoxon-coated glass capillaries. Plasma was collected and measured for triglyceride (TG) and total cholesterol (TC) levels. In brief, TG and TC levels were measured using Cobas Triglycerides (106571) and Cobas Total Cholesterol (106570) enzymatic kits (both from Roche Diagnostics, Mannheim, Germany), by adding 200 μL reagent (undiluted for TG and 3× diluted in water for TC) to 7.5 μL 5× diluted samples and incubating at room temperature for 30 min prior to measuring absorption at 492 nm versus 650 nm (for TG) or at 505 nm versus 650 nm (for TC). Plasma high-density lipoprotein cholesterol (HDL-C) and non-HDL-C levels were measured using a previously published approach [34] . Plasma alanine transaminase (ALT) levels were determined at the end of the study using Mouse ALT ELISA Kit (Abcam, Cambridge, MA, USA)
4
Serum Liver Enzyme Analysis
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Serum of mice was collected and the levels of AST and ALT were measured using Mouse AST ELISA kit (ab263882, Abcam) and Mouse ALT ELISA kit (ab282882, Abcam). The absorbance at 450 nm was read with a microplate reader (Bio-Rad).
5
Serum Biomarker Assessment in Mice
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Serum levels of ALT, AST, TNF-α, and IL-6 were detected in line with the kit instructions of the mouse ALT ELISA kit (ab282882, Abcam), mouse AST ELISA kit (ab263882, Abcam), mouse TNF-α ELISA kit (ab208348, Abcam), and mouse IL-6 ELISA Kit (ab222503, Abcam).
6
Evaluating Liver Damage and IL-1β Levels
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Serum alanine aminotransferase 1 (ALT) concentration was determined using an ALT (Mouse) ELISA kit (BioVision Inc., USA) to determine liver damage or injury during the course of BS treatment while IL-1β was measured using IL-1 beta Mouse SimpleStep ELISA kit (Abcam, USA) according to manufacturer’s instructions.
7
Quantifying Hepatocellular Injury by ALT ELISA
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Serum alanine aminotransferase 1 (ALT) concentration was quantified using an ALT (Mouse) ELISA Kit (BioVision Inc., USA) to monitor hepatocellular injury during LA treatment according to manufacturer’s instruction.
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