The identification and quantification of polyphenols from noni fruit and the supernatant from in vitro digestion followed the protocol established by Zhao et al. (2021) (link). UHPLC separation was performed using UPLC-ESI-Q-TRAP-MS (SHIMADZU Nexera X2, Applied Biosystems 4500 QTRAP) equipped with a Waters Acquity UPLC BEH C18 (1.7 μm, 2.1*150 mm). Peak identification was performed in both positive and negative modes. The wavelength was set at 320 nm. The mobile phase A consisted of 0.1 % formic acid in water, and the mobile phase B contained acetonitrile. The column temperature was set to 40 °C, and the auto-sampler temperature was set at 4 °C with an injection volume of 2 μL. The gradient profile was as follows:10 % B (0–0.5 min), 60 % B (0.5–15 min), 98 % B (15–18 min), 10 % B (18–20 min) with a flow rate of 0.3 mL/min. Each sample was injected with a volume of 20 µL. Data acquisition and processing were performed using SCIEX Analyst Work Station Software (Version 1.6.3) and Sciex MultiQuant™3.0.3.
4500 qtrap
Manufactured by Waters Corporation
The 4500 QTRAP is a high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) system designed for qualitative and quantitative analysis. It features a hybrid triple quadrupole linear ion trap mass spectrometer with enhanced scan speed and sensitivity. The 4500 QTRAP is capable of performing a variety of MS-based experiments, including multiple reaction monitoring (MRM), information-dependent acquisition (IDA), and enhanced product ion (EPI) scans.
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Lab products found in correlation
Nexera x2, by Thermo Fisher Scientific (1 mentions)
Analyst work station software, by AB Sciex (1 mentions)
Multiquant 3, by AB Sciex (1 mentions)
Acquity uplc beh c18, by Waters Corporation (1 mentions)
Acquity uplc hss t3 c18, by Waters Corporation (1 mentions)
Cbm30a system, by Thermo Fisher Scientific (1 mentions)
2 protocols using 4500 qtrap
1
Quantitative Analysis of Noni Polyphenols
2
LC-MS/MS Analysis of Metabolite Extracts
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A liquid chromatography (LC)-electrospray ionization (ESI)–MS/MS system (high-performance LC, Shim-pack UFLC Shimadzu CBM30A system; MS, Applied Biosystems 4500 QTRAP) was used to analyze sample extracts, according to the following: Waters ACQUITY UPLC HSS T3 C18 (2.1 × 100 mm, 1.8 μm) column; 40°C; 5 μl injection volume; gradient 100:0 v/v at 0 minutes, 5:95 v/v at 10.0 minutes, 5:95 v/v at 11.0 minutes, 95:5 v/v at 11.1 minutes, and 95:5 v/v at 15.0 minutes. The solvent was water (0.04% acetic acid):acetonitrile (0.04% acetic acid), and the flow rate was 0.4 ml/minute. Alternatively, the effluent was connected to an ESI-triple quadrupole-linear ion trap (QTRAP)-MS. Linear ion trap and triple quadrupole scans were obtained on a QTRAP-MS using an API 4500 QTRAP LC/MS/MS system. According to the metabolites eluted within each period, monitoring of a specific set of multiple reaction-monitoring transitions was conducted.
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