Monoclonal antibodies against abcb1 clone f4

WYE-354 was purchased from MedChemExpress (MCE, Monmouth Junction, NJ, USA). Bovine serum albumin, fetal bovine serum, Dulbecco’s modified Eagle’s Medium, penicillin/streptomycin, and trypsin were purchased from Corning Incorporated (Corning, NY, USA). Verapamil was a product from Enzo Life Sciences (Farmingdale, NY, USA). [³H]-paclitaxel (15 Ci/mmol) was purchased from Moravek Biochemicals, Inc. (Brea, CA, USA). Doxorubicin, paclitaxel, cisplatin, the monoclonal antibodies against ABCB1 (clone F4, Cat # SAB4200775), DMSO, 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT), 4′,6-diamidino-2-phenylindole (DAPI), paraformaldehyde, and Triton X-100, as well as all of the other chemicals, were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG secondary antibody (Cat # 7076S, lot #: 32) was obtained from Cell Signaling Technology Inc. (Danvers, MA, USA). The GAPDH loading control monoclonal antibody (GA1R) (1 mg/mL, Cat # MA5-15738, lot #: SA247966), Alexa Fluor 488 conjugated goat anti-mouse IgG cross-adsorbed secondary antibody (2 mg/mL, Cat # A32723) were obtained from Thermo Fisher Scientific Inc. (Rockford, IL, USA).

ARS-1620 was a free sample from ChemieTek (Indianapolis, IN). The monoclonal antibodies against ABCB1 (clone F4, Cat # SAB4200775) and other chemicals were purchased from Sigma Chemical Co (St. Louis, MO) except otherwise specified. Antibiotics (penicillin/streptomycin), trypsin-EDTA, and fetal bovine serum (FBS) were obtained from Hyclone (Waltham, MA, United States). The Horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG secondary antibody (Cat # 7076S, lot #: 32) was the product of Cell Signaling Technology Inc. (Danvers, MA, United States). The GAPDH loading control monoclonal antibody (GA1R) (Cat # MA5-15738, lot #: SA247966), Alexa Fluor 488 conjugated goat anti-mouse IgG cross-adsorbed secondary antibody (Cat # A32723) were acquired from Thermo Fisher Scientific Inc. (Rockford, IL, United States). [³H]-paclitaxel (15 Ci/mmol) was obtained from Moravek Biochemicals, Inc. (Brea, CA).