Rpmi 1640 and dmem medium

Manufactured by Thermo Fisher Scientific

Sourced in United States

RPMI-1640 and DMEM are commonly used cell culture media. RPMI-1640 is a buffered, liquid medium that supports the growth of a wide variety of cells, including human and other mammalian cells. DMEM is another widely used cell culture medium that provides a balanced salt solution, amino acids, vitamins, and other components necessary for the growth and maintenance of many cell types.

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4 protocols using rpmi 1640 and dmem medium

Synthesis and Characterization of Iron-based Compounds

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Potassium hydroxide (KOH) was obtained from Merck (Darmstadt, Germany). Hydrochloric acid (HCl) was purchased from Sigma-Aldrich (Rio de Janeiro, Brazil). Ferric dichloride tetrahydrate (FeCl₂•4H₂O) (reagent grade, p.a., ≥99%) was obtained from J.T. Baker, Inc. (Phillipsburg, NJ, USA). Ferric trichloride hexahydrate (FeCl₃•6H₂O) (reagent grade, p.a., ≥97%) and Tetraethylammonium hydroxide ((C₂H₅)₄N(OH)) as an aqueous solution 20% wt) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). All reagents were used as given without further purification. These materials were used for the reference synthesis [45 (link)]. Commercial turmeric powder from India (TRS, Curcuma longa L. Powder) was used in this study. Deionized water and ethanol were used in both experiments.
Chemicals such as dimethyl sulfoxide (DMSO) (Cat. D2650; 100% purity), Neutral red (NR), and 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide tetrazolium (MTT) were obtained from Sigma Chemical Co. (St. Louis, MO, USA); RPMI-1640 and DMEM medium, Fetal bovine serum (FBS), phytohemagglutinin, nonessential amino acids (100 mM), L-glutamine, sodium pyruvate, and antimycotic solutions were obtained from Invitrogen-Gibco (Carlsbad, CA, USA). Other reagents were obtained from J.T. Baker, Inc. (Deventer, the Netherlands), as indicated.

Alvarado-Noguez M.L., Matías-Reyes A.E., Pérez-González M., Tomás S.A., Hernández-Aguilar C., Domínguez-Pacheco F.A., Arenas-Alatorre J.A., Cruz-Orea A., Carbajal-Tinoco M.D., Galot-Linaldi J., Estrada-Muñiz E., Vega-Loyo L, & Santoyo-Salazar J. (2023). Processing and Physicochemical Properties of Magnetite Nanoparticles Coated with Curcuma longa L. Extract. Materials, 16(8), 3020.

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Synthesis of PTX-VE and 5-FU-TPGS for Cancer Treatment

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PTX was purchased from
Lc Laboratories (Wobum, MA). PTX–VE and 5-FU–TPGS were
synthesized in our lab (Figure 2). The synthesis
and characterization of PTX–VE and 5-FU–TPGS is provided
in the Supporting Information (Figures
S1–S4). Paclitaxel injection (Taxol) was manufactured by Ben
Venue Laboratories, Inc. (Bedford, OH). 5-Fluorouracil injection
was purchased from APP Pharmaceuticals, LLC (Schaumburg, IL). Antibodies
against p-53, β-tubulin, P-gp, GAPDH, and horseradish peroxidase
(HRP)-conjugated anti-mouse or anti-rabbit whole IgG were obtained
from Santa Cruz Biotechnology (San Diego, CA). DeadEnd Fluorometric
TUNEL assay kits were obtained from Promega (Madison, WI). Other chemicals
were purchased from Sigma-Aldrich (St. Louis, MO).
KB-3-1 and KB-8-5 cells originally obtained from American
Type
Culture Collection (ATCC) (Manassas, VA) were maintained in RPMI 1640
and DMEM medium (Invitrogen, Carlsbad, CA) containing 10% fetal bovine
serum (Invitrogen, Carlsbad, CA), 100 unit/mL penicillin, and 100
μg/mL streptomycin (Invitrogen, Carlsbad, CA). Cells were cultivated
in a humidified incubator at 37 °C and 5% CO₂.
Mice were purchased from the National Cancer Institute (Bethesda,
MD). All experiments performed on animals were in accordance with
and approved by the Institutional Animal Care and Use Committee at
the University of North Carolina at Chapel Hill.

Ma Y., Liu D., Wang D., Wang Y., Fu Q., Fallon J.K., Yang X., He Z, & Liu F. (2014). Combinational Delivery of Hydrophobic and Hydrophilic Anticancer Drugs in Single Nanoemulsions To Treat MDR in Cancer. Molecular Pharmaceutics, 11(8), 2623-2630.

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Molecular Mechanism of Metabolic Regulators

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Reagents were purchased from the following manufacturers: DMEM and RPMI 1640 Medium from Thermo Fisher Gibco, GlutaMAX Supplement from Thermo Fisher Gibco; Fetal Bovine Serum (FBS) and from Cytiva HyClone; Transit LT-1 reagent (cat. # MIR 2300) from Mirus Bio; Polyethylenimine linear MW 25000 transfection grade (PEI 25K) (cat. # 239661) from Polysciences Inc.; from Millipore Sigma; from Cayman Chemical; Bolt 4-12% Bis-Tris gels, Halt Protease Inhibitor Cocktail from Invitrogen; Bradford Reagent from Bio-Rad; from Cell Signaling Technology; Chamelon Duo Pre-Stained Ladder, IRDye 800CW and IRDye 680RD secondary antibody from Li-COR; Cell-titer Glo (cat. # G7572) from Promega; NEBNext Ultra II Q5 Master Mix (cat. # M0544L), BstXI (cat. # R0113L), Blp I (cat. # R0585L) from New England Biolabs; NucleoSpin Blood DNA isolation kit (cat. # 740950.50) from Macherey Nagel; Human Genome-wide CRISPRa-v2 Libraries were provided by Jonathan Weissman (UCSF) via Addgene (cat. # 1000000091).
BIOIO-1001 and BIOIO-1002 were synthesized by Dipharma, Inc (Kalamazoo, MI). BIOIO-1001 and BIOIO-1002 were licensed from Metabolic Solutions Development Company (MSDC, Kalamazoo, MI). UK-5099 and Pioglitazone was purchased from Sigma Chemical Co. (St. Louis, MO).

Li J., Kumar S., Miachin K., Bean N.L., Halawi O., Lee S., Park J., Pierre T.H., Hor J.H., Ng S.Y., Wallace K.J., Rindtorff N., Miller T.M., Niehoff M.L., Farr S.A., Kletzien R.F., Colca J., Tanis S.P., Chen Y., Griffett K., McCommis K.S., Finck B.N, & Peterson T.R. (2023). A DUAL MTOR/NAD+ ACTING GEROTHERAPY. bioRxiv.

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Evaluating Cell Culture Conditions

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Each chemical and reagent utilized in the present work was analytically pure. The
reference substances had the purity > 98%. cAMP and bovine serum albumin (BSA) were
provided by Sigma-Aldrich (Shanghai). Cells lines (epithelial cell line BEAS-2B and
macrophages cell line THP-1) were obtained from the American-type culture collection
(ATCC) and cultured in a specific medium (DMEM and RPMI 1640 medium, Gibco) that contained
10% fetal bovine serum (FBS) with or without antibiotics in a humid incubator under 5%
CO₂ and 37°C conditions.

Qin M., Guo A., Li F., Zhang F., Bi M., Zhang Y, & Zhu W. (2021). Liquiritigenin enhances cyclic adenosine monophosphate production to mitigate inflammation in dendritic cells. International Journal of Immunopathology and Pharmacology, 35, 20587384211038098.

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