Eclipse e200mv r

Clinical assessment consisted of a questionnaire on present or previous symptoms of UGS, known bladder or kidney disease, previous praziquantel treatment, the duration of fresh water contact and pregnancy. Three urine samples, provided between 10:00 h and 14:00 h on three consecutive days, were requested. Semiquantitative urine dipstick testing as well as urine filtration (10 ml urine) for microscopy for the presence of Schistosoma haematobium were performed (Dipstick: Combur 10 Test, Roche Diagnostics Ltd, Risch, Switzerland; Millipore filter: Nuclepore Track-Etch Membrane Filtration Products, Whatman, Maidstone, UK; microscope: Eclipse E200MV R, Nikon, Tokyo, Japan). A positive result was defined as any detection of eggs in the filtered urine. Heavy intensity infection was defined as ≥50 eggs/10 ml urine. 21 In addition, 10 ml of urine was centrifuged for 5 min at 710 x g and, after discarding the supernatant, 500 μl of urine was stored at -20 • C for Schistosoma genus PCR. In the case of negative microscopy, urine real-time PCR for Schistosoma-specific DNA was performed at CERMEL, as published previously. 22

Morphological identification of fungal isolates was performed through macro and microscopic analysis. Initially, the strains were cultivated in PDA culture medium for 7 days at 28 °C. Colony colors and growth rates were evaluated with the aid of a stereoscope (NIKON SMZ 745 Model C -LEDS -China). The presence and size of sclerotia and conidia morphology were evaluated by the staining method on lactophenol slides with the aid of an optical microscope (NIKON Eclipse E200MVR -China).