Fluorescence, brightfield, and polarized light microscopy were performed via use of an Olympus BX50 fluorescence microscope (mercury source), equipped with a BX-FLA reflected light attachment. Lumogallion fluorescence was collected through an Olympus U-MNIB3 fluorescence filter cube (excitation filter: 470-495 nm, dichromatic mirror: 505 nm, longpass emission filter: 510 nm) and ThS fluorescence collected by use of an Olympus U-MWBV2 cube (excitation filter: 400-440 nm, dichromatic mirror: 455 nm, longpass emission filter: 475 nm). Polarized light illumination was achieved by use of a U-POT drop-in polarizer and a U-ANT transmitted light analyzer (both from Olympus, UK). Images were acquired by use of a ColorView III charged coupled device (CCD) camera and the CellD software suite (Olympus, SiS Imaging Solutions, GmbH). Fluorescence channels were merged using Photoshop (Adobe Systems Inc., USA).
U mnib3 fluorescence filter cube
Manufactured by Olympus
Sourced in United Kingdom
The U-MNIB3 fluorescence filter cube is a component used in fluorescence microscopy systems. It is designed to isolate and transmit specific wavelengths of light for fluorescence excitation and emission, enabling the visualization and analysis of fluorescently labeled samples.
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Lab products found in correlation
2 protocols using u mnib3 fluorescence filter cube
1
Multimodal Microscopy for Biomolecular Imaging
2
Lumogallion Fluorescence Imaging Protocol
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Brightfield and fluorescence micrographs were acquired using an Olympus BX50 fluorescence mic-roscope (mercury source) equipped with a vertical illuminator and BX-FLA reflected light attachment. A U-MNIB3 fluorescence filter cube (bandpassλex: 470–495 nm, dichromatic mirror: 505 nm, longp-assλem: 510 nm) was used to acquire lumogallion fluorescence (Olympus, UK). Images were captured using an Olympus DP74 (CMOS processor) color camera and the OLYMPUS cellSens (Standard 3.1) software suite. Optimal exposure times for collecting lumogallion fluorescence were set automatically by the superfluorescence (SFL) mode of the Olympus DP74 camera. The same optimized exposure settings were then subsequently fixed for each respective and corresponding autofluorescence micrograph. Merging of fluorescence and brightfield channels was achieved using Photoshop (Adobe Systems Inc., US).
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