PCR primers were designed with Primer3 Plus Bioinformatics Software (version 3.3.0) and NCBI BLAST and purchased from Eurofins Genomics, Wolverhampton, UK. Selected primers had a low probability of primer dimer or secondary structure formation, GC% of 50–65%, and melting temperature between 55 and 60 °C.
Ncbi blast
Manufactured by Eurofins
Sourced in United Kingdom
NCBI BLAST is a software tool developed by the National Center for Biotechnology Information (NCBI) that allows users to perform sequence alignment searches against various biological sequence databases. It is a widely used bioinformatics tool that enables researchers to compare a query sequence with a database of known sequences to identify similar or homologous sequences.
Automatically generated - may contain errors
Lab products found in correlation
Protein ladders, by Santa Cruz Biotechnology (1 mentions)
Power sybr green, by Thermo Fisher Scientific (1 mentions)
Bca assay kit, by Merck Group (1 mentions)
Pre designed sirna, by Thermo Fisher Scientific (1 mentions)
Polyplus interferin, by Source Bioscience (1 mentions)
Protein a g agarose gel beads, by Santa Cruz Biotechnology (1 mentions)
Rna to cdna kit, by Thermo Fisher Scientific (1 mentions)
Dmem culture medium, by Lonza (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
2 protocols using ncbi blast
1
Primer Design for Molecular Analysis
2
Protein expression and gene silencing protocol
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Chemicals reagents used to prepare buffers and BCA Assay kit were purchased from Sigma Aldrich, UK and Fisher Scientific, UK. Buffers used include RIPA buffer, Phosphate-buffered saline (PBS), Tris-buffered saline (TBS), Blocking Buffer, Cell lysis buffer, elution buffer, SDS sample buffer, and ECL detection reagent [23] . PolyPlus INTERFERin was purchased from Source Bioscience, UK. Pre-designed siRNA, Opti-MEM, Power SYBR Green, RNa to cDNA kit, and gel casting materials were purchased from Life Technologies, UK.
Antibodies, Protein A/G agarose gel beads, and protein ladders were obtained from Santa Cruz Biotechnology, UK and Cell Signalling Technologies, UK. DMEM culture medium and other cell culture materials were purchased from Lonza, UK. PCR primers were designed with Primer3 Plus Bioinformatics Softwaew and NCBI BLAST, and purchased from Eurofins Genomics. The cells were incubated at 37ºC, 5% CO2 with Opti-MEM for 24 hours for efficient gene silencing.
Antibodies, Protein A/G agarose gel beads, and protein ladders were obtained from Santa Cruz Biotechnology, UK and Cell Signalling Technologies, UK. DMEM culture medium and other cell culture materials were purchased from Lonza, UK. PCR primers were designed with Primer3 Plus Bioinformatics Softwaew and NCBI BLAST, and purchased from Eurofins Genomics. The cells were incubated at 37ºC, 5% CO2 with Opti-MEM for 24 hours for efficient gene silencing.
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