Short hairpin RNA (shRNA) targeted YTHDF2, MAGI1-IT1, METTL3, IL6R, or scrambled oligonucleotides, purchased from GenePharma (Shanghai, China) were inserted into pGLVH1 vector (sh-YTHDF2, sh-MAGI1-IT1, sh-METTL3, sh-IL6R). The full length of FOXO3, MAGI1-IT1 coding sequence was amplified and cloned into a pcDNA3.1 (Invitrogen, Carlsbad, CA, USA) vector (Oe-FOXO3, Oe-MAGI1-IT1). miR-664b-3p inhibitor were purchased from RiboBio (Guangzhou, China). The plasmids mentioned above were transfected into cells by Lipofectamine 3000 (Invitrogen).
Ythdf2
Manufactured by GenePharma
Sourced in China
YTHDF2 is a laboratory equipment product that serves a core function in genetic research and analysis. It is designed to perform specific tasks related to the study of RNA-binding proteins and their roles in cellular processes. The details of its functionality and intended applications are best provided by the product's technical specifications or by consulting with the manufacturer.
Automatically generated - may contain errors
Lab products found in correlation
Sh ythdf2, by GenePharma (1 mentions)
Mettl3, by GenePharma (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Pcdna3, by Thermo Fisher Scientific (1 mentions)
Sh mettl3, by GenePharma (1 mentions)
Ythdc1, by GenePharma (1 mentions)
Ythdf1, by GenePharma (1 mentions)
X tremegenetm sirna transfection reagent, by Roche (1 mentions)
X tremegene hp dna transfection reagent, by Roche (1 mentions)
2 protocols using ythdf2
1
Genetic Manipulation via Plasmid Transfection
2
Gene Knockdown by RNAi Oligos
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For knockdown of gene expression, sets of three synthesized duplex RNAi oligos targeting human TTC22, WTAP, YTHDF1, YTHDF2, YTHDF3 or YTHDC1 mRNA were purchased from Gene Pharma (Shanghai, China; Table S1). Two siRNAs for RPL4 were purchased from RuiBo Bio (stB0001897A for siRPL4#1 and stB0001897B for siRPL4#2; Guangzhou, China). The LV3(H1/GFP& Puro)-shWTAP and empty vector plasmids were also constructed by GenePharma (Shanghai, China). Lentivirus particles of pLV-hU6-shRPL4 and the negative control were from SyngenTech Co., Ltd (pHS-ASR-1219 and pHS-ASR-LW429, Beijing, China). Cells at 70-80% con uence were transfected with siRNAs using X-tremeGENETM siRNA Transfection Reagent or with plasmids using X-tremeGENE HP DNA Transfection Reagent (Roche, Mannheim, Germany) according to the manufacturer's instructions. Successful knockdown of the target gene was con rmed by Western blot and qRT-PCR analyses. Scrambled siRNA sequences were used as negative controls.
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