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Anti lats1 antibody

Manufactured by Cell Signaling Technology

The Anti-LATS1 antibody is a laboratory reagent used to detect and analyze the LATS1 protein in biological samples. LATS1 is a key regulator of the Hippo signaling pathway, which plays a critical role in cell growth, proliferation, and organ size control. This antibody can be used in various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of LATS1 in different cell types and tissues.

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2 protocols using anti lats1 antibody

1

Dissecting YAP/TAZ Signaling Pathways

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Anti-YAP/TAZ (D24E4) dual antibodies were used at 1:1000 (Cell Signaling Technologies, catalog# 8418S), anti-YAP (D8H1X) antibody used at 1:1000 (Cell Signaling Technologies catalog# 14074T), anti-phosphorylated YAP S127 at 1:1000 (Cell Signaling Technologies, catalog# 4911), anti-TAZ (Novus, catalog# NB110-58359), anti-phosphorylated TAZ S89 (E1X9C) at 1:1000 (Cell Signaling Technologies, catalog# 59971), anti-BZLF1 antibody was used at 1:500 (Santa Cruz, catalog# sc-53904), anti-BRLF1 antibody at 1:2000 (isolated from rabbits injected with peptide sequence EDPDEETSSQAVKALREMAD), anti-BMRF1 antibody at 1:2000 (Millipore-Sigma, catalog# MAB8186), anti-LATS1 antibody at 1:1000 (Cell Signaling Technologies, catalog# 9153), anti-phosphorylated LATS S909 at 1:1000 (Cell Signaling Technologies catalog# 9157), PAN-TEAD antibodies at 1:1000 (Cell Signaling Technologies catalog# 13295S), anti-tubulin antibody at 1:4000 (Sigma, catalog# T5168), anti-β actin antibody at 1:5000 (Sigma catalog# 5441), and anti-HSP90 (F8) at 1:1000 (Santa Cruz catalog# sc-13119). The secondary antibodies used were Horseradish peroxide (HRP)- labeled goat anti-mouse antibody at 1:5000 (Thermo Scientific catalog# 31430), HRP- labeled donkey anti-goat antibody (Santa Cruz catalog# sc-2056, 1:5000), and HRP- labeled anti-rabbit antibody (Fisher Scientific catalog# 31460 1:10000).
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2

Apoptosis and Necroptosis Signaling Pathways

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Chaetocin (Cat. HY-N2019-10 mg), N-acetyl-L-cysteine (NAC) (Cat. HY-B0215-500 mg), XMU-MP-1 (Cat. HY-100526-5 mg), z-VAD-fmk (Cat. HY-16658B-5 mg) and Necrostatin-1 (Cat. HY-15760-10 mg) were purchased from MedChemExpress (Shanghai, China). The anti-p-Histone h3 antibody (Cat. ET1601-30), anti-PARP antibody (Cat. ET1608-56), anti-Cleaved PARP antibody (Cat. ET1608-10), anti-Caspase-3 antibody (Cat. ET1608-64), anti-Cleaved-Caspase-3 antibody (Cat. ET1608-64), anti-Bcl-2 antibody (Cat. ET1702-53) and anti-Bax antibody (Cat. ET1603-34) were purchased from HuaBio (Hangzhou, China). The anti-p-CDK1 (Thr161) antibody (Cat. BS-3481R) was purchased from BIOSS (Beijing, China). The anti-MST1 antibody (Cat. 3682), anti-MST2 antibody (Cat. 3952), anti-p-MST1 (Thr183)/MST2 (Thr180) antibody (Cat. bs-3294R), anti-LATS1 antibody (Cat. 3477), anti-p-LATS1 (Ser909) antibody (Cat. bs-3246R), anti-p-LATS1 (Thr1079) antibody (Cat. bs-3245R), anti-MOB1 antibody (Cat. 13730), anti-p-MOB1 (Thr35) antibody (Cat. 8699), anti-SAV1 antibody (Cat. 13301), anti-YAP antibody (Cat. 14074), anti-p-YAP (Ser127) antibody (Cat. 13008), anti-p-YAP (Ser397) antibody (Cat. 13619) and GAPDH (Cat. 5174) were purchased from Cell Signaling Technology (Danvers, MA, USA).
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