Immunoprecipitation was performed as described in previous study [23] . The MCF-7 cells total cell lysis was pre-cleared with rabbit IgG for 2 h and subsequently immunoprecipitated with TRIM3 antibody (HAP043396, Sigma) over night, while rabbit IgG (Santa Cruz) was used as the negative control. The bounded protein was analyzed by Anti-P53 antibody (SC-126, Santa Cruz). For the domain CoIP assay, the P53 or TRIM3 variants were transfected into HEK293 cells. The total cell lysis was pre-cleared with rabbit IgG for 2 hours and subsequently immunoprecipitated with Myc antibody overnight, while the rabbit IgG was used as the negative control. The bounded protein was analyzed via GFP antibody (Ab290, Abcam).
Trim3 antibody hap043396
Manufactured by Merck Group
The TRIM3 antibody (HAP043396) is a laboratory reagent used for the detection and analysis of the TRIM3 protein in biological samples. TRIM3 is a member of the tripartite motif (TRIM) family of proteins that play a role in various cellular processes. This antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to investigate the expression and localization of TRIM3 in research settings.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using trim3 antibody hap043396
1
TRIM3 and p53 Co-immunoprecipitation
2
TRIM3 and p53 Co-immunoprecipitation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunoprecipitation was performed as described in previous study [23] . The MCF-7 cells total cell lysis was pre-cleared with rabbit IgG for 2 h and subsequently immunoprecipitated with TRIM3 antibody (HAP043396, Sigma) over night, while rabbit IgG (Santa Cruz) was used as the negative control. The bounded protein was analyzed by Anti-P53 antibody (SC-126, Santa Cruz). For the domain CoIP assay, the P53 or TRIM3 variants were transfected into HEK293 cells. The total cell lysis was pre-cleared with rabbit IgG for 2 hours and subsequently immunoprecipitated with Myc antibody overnight, while the rabbit IgG was used as the negative control. The bounded protein was analyzed via GFP antibody (Ab290, Abcam).
3
TRIM3 and p53 Co-immunoprecipitation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunoprecipitation was performed as described in previous study [23] . The MCF-7 cells total cell lysis was pre-cleared with rabbit IgG for 2 h and subsequently immunoprecipitated with TRIM3 antibody (HAP043396, Sigma) over night, while rabbit IgG (Santa Cruz) was used as the negative control. The bounded protein was analyzed by Anti-P53 antibody (SC-126, Santa Cruz). For the domain CoIP assay, the P53 or TRIM3 variants were transfected into HEK293 cells. The total cell lysis was pre-cleared with rabbit IgG for 2 hours and subsequently immunoprecipitated with Myc antibody overnight, while the rabbit IgG was used as the negative control. The bounded protein was analyzed via GFP antibody (Ab290, Abcam).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!