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2 protocols using pheophorbide a

1

Lipid Membrane Characterization Protocol

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Poly (ethylene oxide)-b-poly (caprolactone) (PEO-PCL) 5k-4k, poly (ethylene oxide)-b-poly (styrene) (PEO-PS) 2,3k-3,1k were purchased from Polymer Source (Polymer Source, Inc., Dorval, Canada). 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N- (lissamine rhodamine B sulphonyl) (ammonium salt) (Lys- Rhod-PE) come from Avanti Polar Lipids (Avanti Polar Lipids, Inc., USA). (N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt) was bought from Thermo Fisher (NBD-PE, N360, Waltham, MA, US). Pheophorbide-a was purchased from Wako (Japan). Chloroform, methanol, acetone, phosphate buffer (PBS, P4417) and carboxyfluorescein (CBF, 21877) were purchased from Sigma Aldrich (Merck, Darmstadt, Germany). The water used to prepare all PBS solutions was ultrapure water at 18 MΩ resistivity obtained with an ELGA Purelab Flex system (ELGA LabWater, High Wycombe, UK). Anthracene-9,10-dipropionic acid disodium salt (ADPA) was purchased from Santa Cruz Biotechnology (Dallas, TX, US).
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2

Extraction and Purification Protocol

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N,N-dimethylformamide (DMF) PAR grade and LC/MS grade solvents and water were supplied by Panreac (Barcelona, Spain), while acetone HPLC grade was supplied by Merck. The deionised water used was obtained from a Milli-Q 50 system (Millipore Corp., Milford, MA, USA) . Sodium chloride, α-amylase (porcine pancreas, VI-B), pepsin (porcine), bile extract (porcine), lipase pancreatic (porcine), tetrabutylammonium acetate, ammonium acetate (98%), and butylated hydroxytoluene (BHT) were provided by Sigma-Aldrich Chemical Co. (Madrid, Spain) . Pheophorbide a and chlorophyll a were purchased from Wako Chemicals (Tokyo, Japan). Other reagents (acetone andpotassium chloride analysis grade) were supplied by Teknokroma (Barcelona, Spain).
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