Telfa non adherent dressings

Adult age- and sex-matched male and female Prrx1^Cre; R26^mTmG mice were used for cutaneous wound healing experiments. Splinted, full-thickness excisional wounds were performed as previously described (Rinkevich et al., 2015 (link)). In brief, mice were induced and maintained under anesthesia using a 2% isoflurane/oxygen mixture at 3 L per minute. Ventral hair was removed with a chemical depilatory cream and skin was prepped with povidone-iodine and alcohol. Two 6-mm full-thickness circular wound were placed through the panniculus carnosus; one at the upper abdomen of each animal, directly caudal to the xyphoid process, and one immediately caudal. A circular silicone 12-mm diameter stent was secured around the perimeter of each wound with glue and 8 simple interrupted 6–0 nylon sutures (Ethicon, Somerville, New Jersey). Wounds were dressed using Tegaderm (3M, Minnesota, USA) and bolstered by Telfa non-adherent dressings (Covidien, Dublin, Republic of Ireland). Dressings were changed every other day under anesthesia until wounds had fully healed. Wounds were imaged every other day, and wound healing curves were plotted as a percentage of wound size versus days since wounding. The relative proportions of PPF and PNF subpopulations throughout wounding (POD 4, 7, 9, 14) were analyzed by FACs.