Cells were trypsinized and pelleted by centrifugation. After washing twice in phosphate-buffered saline (PBS), the cells were resuspended in serum-free DMEM at a density of 8 × 105 cells/ml, and 200 μl of the cell suspension was seeded onto the basement Matrigel-coated membrane matrix (BD Biosciences, San Jose, CA, USA). FBS was added to the lower chamber as a chemoattractant. After 20 h, the noninvading cells were gently removed with a cotton swab. Invasive cells located on the lower side of the chamber were fixed with 4% paraformaldehyde (PFA; Sigma-Aldrich) for 20 min at room temperature prior to crystal violet staining. Three independent visual fields were examined via microscopic observation, and the number of cells was determined.
Basement matrigel coated membrane matrix
Manufactured by BD
Sourced in United States
Basement Matrigel-coated membrane matrix is a laboratory product that serves as a substrate for cell culture. It is a complex mixture of extracellular matrix proteins, growth factors, and other components derived from a mouse sarcoma cell line. The matrix provides a physiologically relevant environment for the growth and differentiation of various cell types.
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Lab products found in correlation
2 protocols using basement matrigel coated membrane matrix
1
Transwell Invasion Assay Protocol
2
Cell Invasion Assay on Matrigel
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Cells were trypsinized and pelleted by centrifugation. After washing twice in phosphate buffered saline (PBS buffer), the cells were resuspended in serum-free DMEM medium at a density of 4 × 105 cells/ml, and 200 μl of the cell suspension was seeded onto the basement Matrigel-coated membrane matrix (BD Biosiences). Fetal bovine serum was added to the lower chamber as a chemoattractant. After 20 hours, the noninvading cells were gently removed with a cotton swab. Invasive cells located on the lower side of the chamber were fixed with 4% paraformaldehyde (PFA) for 20 minutes at room temperature prior to Crystal Violet (C01201, Beyotime) staining. Three independent visual fields were examined via microscopic observation, and the number of cells was determined.
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