Apc anti ifnγ

Manufactured by Thermo Fisher Scientific

The APC-anti-IFNγ is a fluorescently labeled antibody that binds to the interferon-gamma (IFN-γ) protein. It is designed for use in flow cytometry applications to detect and quantify IFN-γ-producing cells.

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Lab products found in correlation

Permeabilization buffer, by Thermo Fisher Scientific (1 mentions) Ic fixation buffer, by Thermo Fisher Scientific (1 mentions) Foxp3 fixation permeabilization staining kit, by Thermo Fisher Scientific (1 mentions) Cell stimulation cocktail, by Thermo Fisher Scientific (1 mentions) Anti cd45 fitc, by Thermo Fisher Scientific (1 mentions) Fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Pe cf594 anti cd4, by BD (1 mentions)

Close spelling variants of this product

APC-IFNγ (7 citations) Anti-IFNγ-APC (clone XMG1.2, (4 citations) Anti-mouse IFNγ-APC (clone XMG1.2, (4 citations) APC conjugated anti-mouse IFNγ (3 citations) Anti-mouse IFNγ APC (XMG1.2, (2 citations)

2 protocols using apc anti ifnγ

Intracellular Cytokine Staining Protocol

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Intracellular Foxp3 staining was performed using the Foxp3 fixation/permeabilization staining kit (eBioscience). For ex vivo cultures, cells from the MLN or the LP were stimulated with cell stimulation cocktail (eBioscience) containing PMA, ionomycin, brefeldin A and monensin for 4 hours at 37 °C with 5% CO₂. Following, surface staining with FITC-anti-CD4 (RM4-5) and then intracellular staining with PE-anti-IL-17A (ebio17B7) and APC-anti-IFNγ (XMG1.2) was done using IC fixation buffer and permeabilization buffer (eBioscience).

Chng S.H., Kundu P., Dominguez-Brauer C., Teo W.L., Kawajiri K., Fujii-Kuriyama Y., Mak T.W, & Pettersson S. (2016). Ablating the aryl hydrocarbon receptor (AhR) in CD11c+ cells perturbs intestinal epithelium development and intestinal immunity. Scientific Reports, 6, 23820.

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Isolation and Staining of Intestinal Lymphocytes

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Large intestine was shaken in 5 mM EDTA/1 mM DTT for 10 min and then transferred to fresh 5 mM EDTA/1 mM DTT to incubate for 10 more minutes to obtain IEL fractions. IEL pellets were mixed in 30% isotonic Percoll and layered onto 70% isotonic Percoll. Cells were collected from Percoll gradients following centrifugation at 1800 rpm for 20 min and washed in RPMI-1640. Cells were stained with a combination of the following fluorescence-conjugated mAbs: PE–CF594 anti-CD4 (BD), PE-Cy7 anti-TCRβ (eBioscience), PE anti-CD326 (EpCAM) (Miltenyi Biotec), eFluor450 anti-CD8β (eBioscience), eFluor450 anti-TCRγδ (eBioscience), APC anti-IFNγ (eBioscience), FITC anti-CD45 (eBioscience), PerCP-Cy5.5 anti-CD8α (eBioscience), BV650 anti-IL-17 (BD), APC anti-IL-22 (BioLegend). Dead cells were excluded with Fixable Aqua Dead Cell Stain kit (Invitrogen). Samples were acquired on LSR Fortessa II and analyzed with FlowJo software (v10.0; TreeStar).

Navabi N., Whitt J., Wu S.E., Woo V., Moncivaiz J., Jordan M.B., Vallance B.A., Way S.S, & Alenghat T. (2017). Epithelial histone deacetylase 3 instructs intestinal immunity by coordinating local lymphocyte activation. Cell reports, 19(6), 1165-1175.

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