All NP dispersions showed very weak light scattering and were treated from the photophysical point of view as any solution of molecular species. DLS measurements showed no aggregation of the NPs, even after several months. UV-vis absorption spectra were recorded at 25 °C by means of a Perkin-Elmer Lambda 45 spectrophotometer. Emission spectra and lifetime decay were recorded using a HORIBA Jobin Yvon Fluoromax-4 spectrofluorimeter. Photophysical characterization in the absence of oxygen was carried out on samples of dye solutions and NP dispersions after degassing them by freeze-pump-thaw cycling (P = 10−9 Bar; liquid nitrogen as cooling medium) in a custom made quartz cuvette.
Jobin yvon fluoromax 4 spectrofluorimeter
Manufactured by Horiba
The Jobin Yvon Fluoromax-4 spectrofluorimeter is a laboratory instrument designed for the measurement of fluorescence. It features monochromators for excitation and emission wavelength selection and a photomultiplier tube detector. The Fluoromax-4 is capable of performing steady-state fluorescence measurements.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using jobin yvon fluoromax 4 spectrofluorimeter
1
Photophysical Characterization of Nanoparticles
2
Absorption and Fluorescence Spectroscopy of ITX
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Absorption and fluorescence spectra were recorded at room temperature with an Analytik Jena Specord 210 UV-visible spectrometer and a Horiba Jobin Yvon Fluoromax 4 spectrofluorimeter respectively. Compounds were dissolved in acetonitrile and placed into 1 cm 1 cm quartz cuvette.
The fluorescence quantum yield (Φfluo) of ITX was determined with quinine sulfate dihydrate in 1N H2SO4 as reference (Φfluo = 0.546 38 ), using a conventional procedure. 39 Maximal optical density above 300 nm was maintained below 0.1 in order to avoid inner filter effects. Two excitation wavelengths (360 and 375 nm) and two slit sizes (1 and 2 nm) were used and the results were averaged on these four experiments.
Fluorescence lifetime of ITX was obtained by Time-Correlated Single Photon Counting (TCSPC) using a Horiba Jobin Yvon Fluoromax 4 spectrofluorimeter equipped with a Horiba Jobin Yvon Fluorohub module. Light source was ensured by a Horiba Jobin Yvon 374 nm nano-
The fluorescence quantum yield (Φfluo) of ITX was determined with quinine sulfate dihydrate in 1N H2SO4 as reference (Φfluo = 0.546 38 ), using a conventional procedure. 39 Maximal optical density above 300 nm was maintained below 0.1 in order to avoid inner filter effects. Two excitation wavelengths (360 and 375 nm) and two slit sizes (1 and 2 nm) were used and the results were averaged on these four experiments.
Fluorescence lifetime of ITX was obtained by Time-Correlated Single Photon Counting (TCSPC) using a Horiba Jobin Yvon Fluoromax 4 spectrofluorimeter equipped with a Horiba Jobin Yvon Fluorohub module. Light source was ensured by a Horiba Jobin Yvon 374 nm nano-
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!