Anti trpc6

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-TRPC6 is a laboratory reagent used for the detection and analysis of TRPC6 protein expression in biological samples. It is a specific antibody that binds to the TRPC6 protein, allowing researchers to measure its presence and levels in their experiments.

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TRPC6 (2 citations)

4 protocols using anti trpc6

Antibody Characterization of Ion Channels

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The following primary antibodies were used: anti-PLCγ1 (Santa Cruz, sc-426) for immunocytochemistry and proximity ligation assay experiments; anti-PLCγ2 (Santa Cruz, sc-31751) for immunocytochemistry and proximity ligation assay experiments; anti-PLCβ2 (Santa Cruz, sc-206) for immunocytochemistry and proximity ligation assay experiments; anti-TRPC6 (Alomone, ACC-120) for immunocytochemistry, electrophysiology, and Ca²⁺ imaging experiments; anti-TRPC6 (Santa Cruz, sc-19197) for proximity ligation assay experiments; anti-TRPM4 (Abcam, ab63080) for immunocytochemistry and proximity ligation assay experiments; anti-TRPM4 (Santa Cruz, sc-67125) for immunocytochemistry and proximity ligation assay experiments; and anti-BK_Ca (Alomone, APC-021) for proximity ligation assay experiments. Secondary antibodies used were the following: sheep anti-rabbit Texas Red (Abcam, ab6793-1), bovine anti-rabbit FITC (Santa Cruz, sc-2365), donkey anti-goat Texas Red (Santa Cruz, sc-2783), and donkey anti-goat FITC (Abcam, ab6881).

Gonzales A.L., Yang Y., Sullivan M.N., Sanders L., Dabertrand F., Hill-Eubanks D.C., Nelson M.T, & Earley S. (2014). A PLCγ1-Dependent, Force-Sensitive Signaling Network in the Myogenic Constriction of Cerebral Arteries. Science signaling, 7(327), ra49.

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Western Blot Analysis of Kidney Proteins

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Total protein samples were extracted from cultured cells and kidney tissues. Protein concentrations were determined using a BCA protein assay kit (Thermo Fisher Scientific). Samples were separated on a 10% sodium dodecyl sulfate-polyacrylamide gel and transferred onto a polyvinylidene difluoride membrane. The membrane was then blocked overnight at 4 °C with primary antibodies, namely anti-TRPC6 (Santa Cruz, USA), anti-calpain-1 (Abcam, UK), anti-Talin-1 (Abcam), anti-nephrin (Santa Cruz) and anti- calcineurin (Abcam). After washing, the membrane was incubated with goat anti-rabbit IgG (H + L; Jackson ImmunoResearch Laboratories, USA). The membrane was also processed to detect GAPDH (Abcam) for equal loading. The bands recognized by the primary antibody were visualized, and the optical densities of the protein bands were analyzed using TanonImage software (Tanon, China).

Ding Y., Tang X., Wang Y., Yu D., Zhu C, & Yu J. (2021). Tetrandrine alleviates podocyte injury via calcium-dependent calpain-1 signaling blockade. BMC Complementary Medicine and Therapies, 21, 296.

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Antibody Profiling for Cellular Signaling

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In this expreimental study, polyclonal rabbit antibodies,
including anti-Akt, anti-p-Akt, anti-TGFβ, anti-p-PLB,
anti-NFATc, anti-p-NFATc, anti-H1, anti-SERCA2, anti-PMAC4b, anti-NPR-A, anti-TRPC6, and anti-β-Actin,
as well as goat anti-rabbit IgG-HRP (sc-2030) secondary
antibody, were purchased from Santa Cruz Biotechnology,
Inc (Santa Cruz, CA). All other reagents and chemicals
were obtained from Sigma-Aldrich, USA.

Baghaiee B., Bayatmakoo R., Karimi P, & Pescatello L.S. (2021). Moderate Aerobic Training Inhibits Middle-Aged Induced Cardiac Calcineurin-NFAT Signaling by Improving TGF-ß, NPR-A, SERCA2, and TRPC6 in Wistar Rats. Cell Journal (Yakhteh), 23(7), 756-762.

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Western Blot Analysis of Kidney Protein Markers

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Total protein was extracted from MPC5 podocytes and kidney tissues of rats. For sufficient cell lysis, RIPA lysis buffer (Beyotime, China) was added, and then, the supernatant was extracted after centrifugation at 12,000 rpm for 10 min. Protein samples were separated with 10% SDS-PAGE and transferred onto PVDF membranes. Membranes were blocked with skim milk powder and then incubated with primary antibodies to anti-TRPC6 (1 : 1,000; Santa Cruz, USA), RhoA (1 : 1,000; Proteintech, Wuhan, China), ROCK1 (1 : 3,000; Proteintech), synaptopodin (1 : 1,000; Santa Cruz), and GAPDH (1 : 10,000; Abcam, UK). Membranes were then incubated with donkey anti-mouse IgG (H+L; Jackson ImmunoResearch Laboratories, USA) secondary antibody. ECL system (Sharebio, China) was used for band detection and protein bands were analyzed by the Tanon Image software (Tanon, China).

Mao L., Ding Y., Yu D., Yin J, & Yu J. (2022). Tetrandrine Attenuates Podocyte Injury by Inhibiting TRPC6-Mediated RhoA/ROCK1 Pathway. Analytical Cellular Pathology (Amsterdam), 2022, 7534181.

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