The influenza A (H1N1) viruses were incubated with TOF for 1 h at 4 °C, and the mixture was added to RAW 264.7 cells for 2 h at 37 °C. After washing with PBS, the cells were incubated for another 24 h, then the supernatants were collected for the HA assay. Briefly, the supernatant was subjected to a serial two-fold dilution and added to a round-bottom 96-wellplate. The blank medium was used as a negative control. Each sample was mixed with 1% chicken RBCs (Innovative Research, Inc., Southfield, MI, USA) in PBS buffer. After incubation for 1 h at room temperature, the incubated plates were photographed.
Chicken rbcs
Manufactured by Innovative Research
Sourced in United States
1% chicken RBCs are a laboratory reagent consisting of a 1% suspension of red blood cells derived from chicken blood. This product is intended for use in various in vitro assays and experiments that require a source of avian erythrocytes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using chicken rbcs
1
Hemagglutination Assay for Influenza A (H1N1)
2
Hemagglutination Inhibition Assay for Influenza
Check if the same lab product or an alternative is used in the 5 most similar protocols
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WPS was diluted (1–1000 μg/mL) with PBS in a round-bottomed 96-well plate. The medium was used as a negative control. H1N1, H1N1-GFP, or H3N2 (64 HAUs in PBS) was added to each well and incubated for 30 min at 37°C under 5% CO2. Each sample was mixed with 1% chicken RBCs (Innovative Research, Inc., Southfield, MI, USA) in PBS. After incubation for 1 h at room temperature, plates were photographed.
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