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Vacuum filtration assembly

Manufactured by Merck Group

Vacuum filtration assembly is a laboratory equipment used for separating solid particles from a liquid by applying suction. It consists of a filter funnel, a filter flask, and a vacuum source. The assembly creates a pressure differential that pulls the liquid through the filter, leaving the solid particles behind. This equipment is commonly used in various laboratory procedures, such as sample preparation, purification, and analysis.

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2 protocols using vacuum filtration assembly

1

Fabrication of Nacre-like SNF/HAP:CNF Membranes

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All of the nacre-like SNF/HAP:CNF membranes were fabricated by vacuum filtration of the SNF/HAP:CNF dispersions through a Sigma-Aldrich vacuum filtration assembly and polycarbonate filtration membranes (pore size, 200 nm; diameter 47 mm; Sigma-Aldrich). After drying, the membranes were easily removed from the support substrate. For the preparation of random distributed SNF/HAP:CNF membranes, the well-mixed SNF/HAP:CNF solution was cast onto a polyethylene plate and allowed to dry at ~25°C and 50% relative humidity to give membranes of an approximate thickness of 10 μm. The SEM tests confirmed no multilayer structure is in these membranes.
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2

Analytical Characterization of Pharmaceutical Samples

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Analytical balance (Shimadzu, Kyoto, Japan); bath sonicator (Hwashin Technology Co., Yeongcheon-si, Republic of Korea); vacuum filtration assembly (Sigma-Aldrich, Darmstadt, Germany); distilled water (Millipore ultrapure water system (Milford, CT, USA)). pH meter (Jenway, Essex, UK), magnetic stirrer (DLAB, Fontana, CA, USA), peristaltic pump (Longer Precision Pump Co., Ltd., Baoding, China), centrifuge (DLAB, CA, USA), scanning electron microscope (JSM-5910, JEOL Ltd., Tokyo, Japan), zeta sizer (Malvern Zetasizer ZS-90, Malvern Instruments Ltd., Malvern, UK), FTIR spectrophotometer (Shimadzu, Kyoto, Japan, IRTracer-100), UV spectrophotometer (Perkin Elmer Series 200, Lambda 25, PerkinElmer, Waltham, MA, USA), Franz diffusion cell (Perme Gear, Hellertown, PA, USA), Simultaneous Thermal Analyzer (STA) 8000 by Perkin Elmer (Waltham, MA, USA). The Perkin Elmer Series 200 HPLC system (Norwalk, CT, USA) is coupled with UV–Visible detector, autosampler, in-line degasser, and column oven. The stationary phase used was SUPLECO C18 column (250 × 4.6 mm, 5 μm) and connected via network chromatography interface (NCI 900). Spring applicator (20 mm dia. × 70 mm L; approx. 1.6 N) was purchased from Micropoint Technologies Pte, Ltd. (Singapore).
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