RB, fetal bovine serum (FBS), penicillin/streptomycin solution, trypsin-EDTA solution, ABDA probe [9,10-antherachenediyl-bis(methylene) dimalonic acid], MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide], and HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) were purchased from Sigma-Aldrich (Taufkirchen, Germany). Dulbecco’s phosphate buffered saline without calcium and magnesium (DPBS) was purchased from Biowest (Nuaillé, France). HBSS (Hanks’ Balanced Salt Solution) and 154 CF culture medium were obtained from Gibco/ThermoFisher Scientific (Waltham, MA, USA). Chelex 100 Resin was obtained from Bio-Rad (Hercules, CA, USA). H2DCFDA (2′,7′ dichlorodihydrofluorescein diacetate) was purchased from Invitrogen/ThermoFisher Scientific (Waltham, MA, USA). Dimethyl sulfoxide (DMSO) was purchased from POCH (Gliwice, Poland). Murine basal cell carcinoma lines (AsZ, BsZ, CsZ) were provided by Dr. Ervin Epstein (Children’s Oakland Research Institute, Oakland, CA, USA).
Organic solvents were dried and freshly distilled under argon prior to use. Chemicals were obtained from commercial sources and used as received. Water solutions of chemicals, amphiphiles and buffer solutions were prepared using milliQ® deionized water. Sonication of amphiphile solutions was done in a Sonorex Super RK 31 H ultrasonic bath (Bandelin Electronic, Berlin, Germany).
Organic solvents were dried and freshly distilled under argon prior to use. Chemicals were obtained from commercial sources and used as received. Water solutions of chemicals, amphiphiles and buffer solutions were prepared using milliQ® deionized water. Sonication of amphiphile solutions was done in a Sonorex Super RK 31 H ultrasonic bath (Bandelin Electronic, Berlin, Germany).