Hanson autoplus multifill collector

Dissolution testing was performed using the Hanson SR-8 Plus^™ Dissolution Test Station (Hanson Research, Los Angeles, CA, USA) by the paddle (United States Pharmacopeia apparatus 2) method at 50 rpm and 37 ± 0.2 °C. The formulations were screened in 500 mL of aqueous-based dissolution media at pH 1.2 (Ph.Eur.9.) and pH 6.8 (Ph.Eur.9.). Pure baicalin and liquid BSNEDDS (equivalent to 5 mg of baicalin) were exposed to the media, and at predetermined time-points, 5 mL of samples were withdrawn and filtered from the media through 5 µm pore size membrane full-flow filters by Hanson AutoPlus Multifill collector (Hanson Research). After every sampling, media replacement was accomplished with 5 mL of fresh buffer solution. Dissolution studies were completed in triplicate and the dissolved drug content (%) ± SD was analyzed by UV-Vis spectrophotometry at 279 nm against equivalent proportions of excipients as blank. Withdrawn and filtered samples were characterized for droplet size and PDI as described for liquid BSNEDDS in Section 2.3. 24 h of visual observation was fulfilled for any precipitation or phase separation. The hydrophobic API favours being dissolved in the droplet of nanoemulsion, and this state (preceded by filtration and dispergation) was measured by UV-Vis spectroscopy.

The dissolution tests were performed using a Hanson SR-8 Plus™ Dissolution Test Station (Hanson Research, Los Angeles, CA, USA) with the paddle (USP 30 dissolution apparatus II) method at a rotation speed of 50 rpm, in 37 ± 0.2 °C medium of 500 mL volume. At predetermined time-points, 5 mL of samples were withdrawn and filtered through 10 µm pore size membrane full-flow filters from the media by Hanson^® AutoPlus Multifill collector (Hanson Research, Los Angeles, CA, USA). After every sampling, media replacement was accomplished with 5 mL of fresh buffer solution. The dissolution tests were performed in triplicates in the instance of every samples.