The reagents were commercially purchased from Sigma-Aldrich (St. Louis, MO, USA) or TCI (Tokyo, Japan). If necessary, solvents were purified and/or dried prior to use. All anhydrous reactions were carried out under a dry atmosphere of nitrogen. Melting points (m.p.) were measured on Thomas-Hoover melting point apparatus (Thomas Scientific, Swedesboro, NJ, USA) and not corrected. 1H, 13C-NMR and HMBC spectra were measured on a Varian 400 MHz spectrometer (Agilent Technologies, Santa Clara, CA, USA) in DMSO-d6, CDCl3, or (CD3)2CO. Chemical shifts (δ) are in ppm relative to tetramethylsilane, and coupling constants (J) are in Hz. DIP-MS (EI) was measured on an Agilent 7890A-5975C GC/MSD (Agilent Technologies). GC/MS (EI) was determined on a SHIMADZU QP 2010 model (Shimadzu, Kyoto, Japan) and FAB-MS was determined on a JEOL JMS-700 Mstation (JEOL, Tokyo, Japan). Fraction collection was performed on an EYELA fraction collector DC-1500 (Tokyo Rikakikai, Tokyo, Japan). An analytical TLC was performed on pre-coated silica gel 60 F254 plates (Merck, Kenilworth, NJ, USA). Solvent systems for TLC and column chromatography were ethyl acetate/n-hexane mixtures and 10% methanol in dichloromethane. Column chromatography was carried out on Merck silica gel 9385 (Merck).
Agilent 7890a gc 5975c msd
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 7890A GC/5975C MSD is a gas chromatograph-mass spectrometer system. It is designed for the separation, identification, and quantification of chemical compounds in complex samples. The 7890A GC provides high-performance gas chromatographic separation, while the 5975C MSD offers sensitive and selective mass spectrometric detection.
Automatically generated - may contain errors
Lab products found in correlation
Thomas hoover melting point apparatus, by Thomas Scientific (1 mentions)
Varian 400 mhz spectrometer, by Agilent Technologies (1 mentions)
Jms 700 mstation, by JEOL (1 mentions)
Model qp 2010, by Shimadzu (1 mentions)
Silica gel 60 f254 plate, by Merck Group (1 mentions)
Hp 5ms capillary column, by Agilent Technologies (1 mentions)
Hi 14k, by Merck Group (1 mentions)
5 protocols using agilent 7890a gc 5975c msd
1
Characterization of Organic Compounds
2
Leaf Volatile Composition Analysis by SPME-GC/MS
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Specimens were prepared using the headspace solid phase microextraction method (Chaintreau et al. 2018) (link). The sample was gently crushed into a 30 mL sample bottle. The solid phase microextraction program was set up. The solid phase microextraction needle was first aged at 250 °C for 30 min at the GC inlet. Then the sample was controlled by the program for automatic solid-phase micro-extraction adsorption, and analyzed by GC/MS.
Instruments, reagents, and chemical analysis: the compositions of volatile chemicals in leaf specimens were analyzed using a gas chromatography-mass spectrometry (Agilent7890A/5975C GC/MSD; Agilent Technologies, Santa Clara, CA, USA) system operating in EI mode, with an HP-5 MS capillary column (30 m × 0.25 mm ID, film thickness 0.25 mm) coupled directly to the MS. The carrier gas was helium, with a flow rate of 1.0 mL/min. The oven temperature proceeded through a program of 50 °C for 1 min, then 50 to 260 °C at 10 °C/min, and subsequently held steady for 15 min. The injector port was held at 250 °C, the detector at 280 °C, and the split ratio was 1:50. Mass spectrometry recordings were taken at 70 eV; scan time 1.5 s; mass range 20 to 625 amu. Chem Station software was used to handle mass spectra and chromatograms. Compounds were identified based on their mass spectra (compared with RTLPEST3.L and NIST05.L).
Instruments, reagents, and chemical analysis: the compositions of volatile chemicals in leaf specimens were analyzed using a gas chromatography-mass spectrometry (Agilent7890A/5975C GC/MSD; Agilent Technologies, Santa Clara, CA, USA) system operating in EI mode, with an HP-5 MS capillary column (30 m × 0.25 mm ID, film thickness 0.25 mm) coupled directly to the MS. The carrier gas was helium, with a flow rate of 1.0 mL/min. The oven temperature proceeded through a program of 50 °C for 1 min, then 50 to 260 °C at 10 °C/min, and subsequently held steady for 15 min. The injector port was held at 250 °C, the detector at 280 °C, and the split ratio was 1:50. Mass spectrometry recordings were taken at 70 eV; scan time 1.5 s; mass range 20 to 625 amu. Chem Station software was used to handle mass spectra and chromatograms. Compounds were identified based on their mass spectra (compared with RTLPEST3.L and NIST05.L).
3
Measurement of Muscle Protein Synthesis
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Plasma glucose and insulin concentrations were analyzed using commercially available kits (ref. no. A11A01667, Glucose HK CP, ABX Diagnostics; and ref. no. HI-14 K, Millipore, respectively). Plasma amino acid concentrations were determined by UPLC-MS, as previously described (26 (link)). Plasma l -[ring-13C6]-phenylalanine enrichments were determined by GC-MS (Agilent 7890A GC/5975C MSD; Agilent Technologies), as previously described (26 (link)).
Basal muscle protein synthesis rates were assessed to confirm that protein ingestion increases muscle protein synthesis rates. The single biopsy approach was applied to assess postabsorptive muscle protein synthesis rates without the need to collect an additional muscle biopsy, as previously described (26 (link), 30 (link)).
Basal muscle protein synthesis rates were assessed to confirm that protein ingestion increases muscle protein synthesis rates. The single biopsy approach was applied to assess postabsorptive muscle protein synthesis rates without the need to collect an additional muscle biopsy, as previously described (26 (link), 30 (link)).
4
Derivatization and TD-GC/MS Analysis of PFIB, COF2, and Phosgene
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The derivatization products of PFIB, COF2, and phosgene were analyzed with TD‐GC/MS using two‐stage desorption on a Turbomatrix 350 ATD‐instrument (Perkin Elmer, Waltham, MA, USA). The following settings were used: primary desorption: 280°C (5 min, 40 ml/min), cold trap desorption: 5−300°C (40°C/min, 3 min hold), valve temperature: 280°C, transfer line temperature: 220°C, inlet split: 40 ml/min, and outlet split: 15 ml/min. The sample was introduced into a GC/MS (Agilent 7890A GC/5975C MSD) at constant column flow rate of 1 ml/min, giving an overall split ratio of 1:32. The temperature of the GC oven (column: DB 5 MS, 30 m × 0.25 mm id × 0.25 µm film thickness) was initially held at 40°C for 2 min, increased to 280°C at 15°C/min, and held for 5 min. The mass spectrometer (EI, 70 eV) was operated in synchronous selected ion monitoring (SIM)/scan acquisition using a full‐scan range of m/z 29−400 and the following SIM ions: m/z 98 (toluene‐d8), m/z 134 (fluorotriethylsilane), m/z 154 and m/z 182 (COF2‐derivative), and m/z 297 and m/z 316 (PFIB derivative).
5
GC-MS Analysis of Oligomer Composition
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The
compositions of the oligomers were measured by GC–mass spectrometry
(MS), and the contents and ratios of the different components were
obtained by an internal standard method. GC–MS analyses were
performed on a PY-7890A-5975C color-mass spectrometer, model Agilent
7890A GC/5975C MSD, using a hydrogen flame ionization detector. The
carrier gas was nitrogen, the column front pressure was 0.07 MPa,
the hydrogen flow rate was 30 mL/min, the carrier gas flow rate was
250 mL/min, the inlet temperature was 250 °C, the detector temperature
was 400 °C, the vaporization chamber temperature was 450 °C,
and the split ratio was 100:1. The temperature was programmed as follows:
the initial temperature was 50 °C, this temperature was maintained
for 10 min, the temperature was increased at 9 °C/min to 380
°C, and this temperature was held for 10 min.
compositions of the oligomers were measured by GC–mass spectrometry
(MS), and the contents and ratios of the different components were
obtained by an internal standard method. GC–MS analyses were
performed on a PY-7890A-5975C color-mass spectrometer, model Agilent
7890A GC/5975C MSD, using a hydrogen flame ionization detector. The
carrier gas was nitrogen, the column front pressure was 0.07 MPa,
the hydrogen flow rate was 30 mL/min, the carrier gas flow rate was
250 mL/min, the inlet temperature was 250 °C, the detector temperature
was 400 °C, the vaporization chamber temperature was 450 °C,
and the split ratio was 100:1. The temperature was programmed as follows:
the initial temperature was 50 °C, this temperature was maintained
for 10 min, the temperature was increased at 9 °C/min to 380
°C, and this temperature was held for 10 min.
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