Anti sars cov 2 np rabbit polyclonal antibody

Manufactured by GeneTex

The anti-SARS-CoV-2-NP rabbit polyclonal antibody is a laboratory reagent that can detect the nucleocapsid protein (NP) of the SARS-CoV-2 virus. It is produced by immunizing rabbits with the SARS-CoV-2 nucleocapsid protein antigen.

Automatically generated - may contain errors

Lab products found in correlation

Dabco glycerol mounting media, by Merck Group (2 mentions) Sp8 laser scanning confocal microscope, by Leica (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions)

Close spelling variants of this product

Anti-NP (9 citations) Anti-NP antibody (3 citations) Rabbit anti-NP polyclonal antibody (2 citations) Rabbit anti-NP antibody (2 citations) Rabbit anti-NP (2 citations)

2 protocols using anti sars cov 2 np rabbit polyclonal antibody

Immunofluorescence Detection of SARS-CoV-2 Nucleocapsid

Check if the same lab product or an alternative is used in the 5 most similar protocols

Placental cells on coverslips were fixed in fixed in 4% paraformaldehyde for 24 hours. Coverslips were blocked and permeabilized in 3% BSA (Sigma) and 0.1 % Triton X-100 (American Bioanalytical). Each sample was incubated overnight with anti-SARS-CoV-2-NP rabbit polyclonal antibody (GeneTex # GTX135357) at a dilution of 1:200. After washing in PBS, coverslips were incubated for 1 hour in a 1:500 dilution of Alexa 594 anti rabbit secondary antibody (Jackson ImmunoResearch 711-585-152), washed again in PBS and treated with 1 μg/mL Hoechst 33342 for 10 min and washed a final time in PBS. Samples were then mounted in DABCO/glycerol mounting media (Sigma) and imaged on a Leica Sp8 Laser Scanning Confocal Microscope equipped with a 40x N.A. 1.3 HC PL APO CS2 objective. Images are displayed as maximum intensity projections of z-stacks and a color bar is given in arbitrary digital units.

Lu-Culligan A., Chavan A.R., Vijayakumar P., Irshaid L., Courchaine E.M., Milano K.M., Tang Z., Pope S.D., Song E., Vogels C.B., Lu-Culligan W.J., Campbell K.H., Casanovas-Massana A., Bermejo S., Toothaker J.M., Lee H.J., Liu F., Schulz W., Fournier J., Muenker M.C., Moore A.J., Konnikova L., Neugebauer K.M., Ring A., Grubaugh N.D., Ko A.I., Morotti R., Guller S., Kliman H.J., Iwasaki A, & Farhadian S.F. (2021). SARS-CoV-2 infection in pregnancy is associated with robust inflammatory response at the maternal-fetal interface. medRxiv.

+ Open protocol

+ Expand

Immunofluorescence Imaging of SARS-CoV-2 Nucleoprotein

Check if the same lab product or an alternative is used in the 5 most similar protocols

Placental cells on coverslips were fixed in fixed in 4% paraformaldehyde for 24 hours. Coverslips were blocked and permeabilized in 3% BSA (Sigma) and 0.1% Triton X-100 (American Bioanalytical). Each sample was incubated overnight with anti-SARS-CoV-2-NP rabbit polyclonal antibody (GeneTex # GTX135357) at a dilution of 1:200. After washing in PBS, coverslips were incubated for 1 hour in a 1:500 dilution of Alexa 594 anti rabbit secondary antibody (Jackson ImmunoResearch 711-585-152), washed again in PBS and treated with 1 μg/mL Hoechst 33342 for 10 min and washed a final time in PBS. Samples were then mounted in DABCO/glycerol mounting media (Sigma) and imaged on a Leica Sp8 Laser Scanning Confocal Microscope equipped with a 40x N.A. 1.3 HC PL APO CS2 objective. Images are displayed as maximum intensity projections of z stacks and a color bar is given in arbitrary digital units.

Lu-Culligan A., Chavan A.R., Vijayakumar P., Irshaid L., Courchaine E.M., Milano K.M., Tang Z., Pope S.D., Song E., Vogels C.B., Lu-Culligan W.J., Campbell K.H., Casanovas-Massana A., Bermejo S., Toothaker J.M., Lee H.J., Liu F., Schulz W., Fournier J., Muenker M.C., Moore A.J., Konnikova L., Neugebauer K.M., Ring A., Grubaugh N.D., Ko A.I., Morotti R., Guller S., Kliman H.J., Iwasaki A, & Farhadian S.F. (2021). Maternal respiratory SARS-CoV-2 infection in pregnancy is associated with a robust inflammatory response at the maternal-fetal interface. Med (New York, N.y.), 2(5), 591-610.e10.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!