Ahdl flex

In KORA F4, total cholesterol (TC) was determined by cholesterol-esterase method (CHOL Flex, Dade-Behring, Germany), triglycerides (TG) and HDLC using the TGL Flex and AHDL Flex method (Dade-Behring), respectively, and LDLC was measured by a direct method (ALDL, Dade-Behring) [29] (link).
All participants were fasting for at least 8 hours. For the present analysis, all participants taking lipid-lowering drugs were excluded. The analysis dataset in KORA F4 is thus based on 2553 participants with available STR measurements and genotypes derived from genome-wide SNP-chips and imputation.
In SAPHIR, blood samples were collected after an overnight fasting period. A complete lipoprotein profile including fasting TC, TG, HDLC and LDLC was determined using routine laboratory procedures (Roche Diagnostics GmbH, Mannheim, Germany). For statistical analysis, all participants taking lipid-lowering drugs were excluded. The analysis dataset in the SAPHIR Study is therefore based on 1648 participants.

Total cholesterol (TC) and HDL-C were measured in fresh serum by enzymatic methods (CHOL Flex and AHDL Flex, Dade Behring, Marburg, Germany). TG was measured in fresh serum with the GPO-PAP method (Dade Behring). HbA1c was quantified with a reverse-phase cation-exchange HPLC method using a Menarini–Arkray Analyser HA-8140 (Menarini Diagnostics, Florence, Italy). Serum glucose levels were assessed by the hexokinase method (GLU Flex, Dade Behring, Marburg, Germany). Serum insulin was determined by ECLIA on a cobas analyser (Roche, Mannheim, Germany).

Four serum lipid measurements (in mg/dl) were collected using the Dimension RxL (Dade Behring); total cholesterol was determined by cholesterol-esterase method (CHOL Flex, Dade-Behring, CHOD-PAP method), HDL-C cholesterol by the AHDL Flex (Dade-Behring, CHOD-PAP method after selective release of HDL-C), LDL-C cholesterol by the ALDL Flex (Dade Behring, CHOD-PAP method after colourless usage of all non-LDL-cholesterol) and triglycerides (TG) by the TGL Flex (Dade Behring, enzymatic colorimetric test, GPO-PAP method).