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2 protocols using ch3oh

1

Metabolite Identification by LC-MS

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Ultra-pure water was generated employing a Milli-Q Integral Water Purification System from Merck Millipore (Darmstadt, Germany). LC–MS grade solvents (CH3CN, isopropanol (IPA), and CH3OH) were acquired from Scharlau (Barcelona, Spain). Formic acid (≥ 95%), and ammonium acetate (≥ 98%) were purchased from Sigma-Aldrich Química SL (Madrid, Spain). Internal standards (ISs) phenylalanine-D5, tryptophan-D3 and caffeine-D9 were purchased from CDN Isotopes (Pointe-Claire, Canada). Valproic acid (VPA) (> 98%), acetaminophen (APAP) (> 99%) and citric acid (CA) (> 99%) were obtained from Sigma-Aldrich (San Luis, MI, USA).
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2

Liquid Chromatography-Mass Spectrometry Materials

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Ultra-pure water was generated employing a Milli-Q Integral Water Purification System from Merck Millipore (Darmstadt, Germany). LC-MS grade solvents (CH 3 CN, isopropanol (IPA), and CH 3 OH) were acquired from Scharlau (Barcelona, Spain). Formic acid (≥95%), and ammonium acetate (≥98%) were purchased from Sigma-Aldrich Química SL (Madrid, Spain). Internal standards (ISs) phenylalanine-D 5 , tryptophan-D 3 and caffeine-D 9 were purchased from CDN Isotopes (Pointe-Claire, Canada). Valproic acid (VPA) (>98%), acetaminophen (APAP) (>99%) and citric acid (CA) (>99%) were obtained from Sigma-Aldrich (San Luis, MI, USA).
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