Smz 2t

Manufactured by Nikon

Sourced in Japan, United States

The Nikon SMZ-2T is a stereomicroscope designed for a wide range of laboratory applications. It provides a magnification range from 0.8x to 5.6x, with a maximum total magnification of 56x. The SMZ-2T features a tilting binocular head and an interpupillary distance adjustment, allowing for comfortable and customized viewing. The instrument is equipped with a built-in 10x eyepiece, and the objective lenses can be easily changed to suit different observation needs.

Automatically generated - may contain errors

Lab products found in correlation

Model lsm 700, by Zeiss (2 mentions) Coolpix 995, by Nikon (2 mentions) α mem medium, by Merck Group (1 mentions) Minimum essential medium (mem), by Merck Group (1 mentions) Ham s f 10, by Merck Group (1 mentions) Human chorionic gonadotropin (hcg), by Merck Group (1 mentions) Model lsm 880, by Zeiss (1 mentions) Mc120 hd, by Leica camera (1 mentions) N4638, by Merck Group (1 mentions) Smz1500, by Leica camera (1 mentions) Tld 18w 08, by Philips (1 mentions) Sylgard 184 silicone elastomer, by Dow (1 mentions) Rotopol 21, by Struers (1 mentions) Carbimet, by Buehler (1 mentions) Accutom 50, by Struers (1 mentions) Malvern mastersizer 3000e hydro, by Malvern Panalytical (1 mentions)

29 protocols using smz 2t

Embryonic Development Morphometric Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Various growth parameters including the primitive streak, the notochord, numbers of somites, and the ECTL were recorded hourly during the first 72 h of development. The ECTL was measured from top of the crown to the tail-bud along the dorsal midline of the embryo. The length of primitive streak was measured from the sickle-shaped region at the interface between the area opaca and area pellucida; such structure can extend to the posterior midline region of the embryo. The length of the notochord was measured from the anterior of Hensen's node to the prosencephalon. The numbers of somite pairs located in the middle of embryonic axis were counted hourly during the entire period of incubation.
The whole mount embryos were photographed using an eyepiece C-mount camera (5 MP linux, Tucsen, China) attached to a trinocular stereomicroscope (SMZ-2T, Nikon, Japan) and processed by the software package Tsview 7 (Bioimager, Canada). Micrographs of embryos were prepared and repositioned with enhanced lighting by using a graphic editing program (Adobe Photoshop, USA).

Lumsangkul C., Fan Y.K., Chang S.C., Ju J.C, & Chiang H.I. (2018). Characterizing early embryonic development of Brown Tsaiya Ducks (Anas platyrhynchos) in comparison with Taiwan Country Chicken (Gallus gallus domestics). PLoS ONE, 13(5), e0196973.

+ Open protocol

+ Expand

Melatonin and Luzindole Effects on Mouse Embryo Development

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Female mice were superovulated by an intra-peritoneal (IP) injection of 7.5 IU pregnant mare serum gonadotropin (PMSG, G 4877, Sigma-Aldrich, USA), followed by injection of 7.5 IU human chorionic gonadotropin (HCG, Karma, Germany) 48 hr later (10 (link)).
To ensure the reliability of gestation time, female mice were paired overnight with males of proven fertility (ratio = 1:1) and females with a vaginal plug were selected for the study.
At 42-48 hr after the HCG injection, the embryos were flushed into Ham’s F10 (Sigma, USA) medium under a stereomicroscope (Nikon SMZ-2T, Japan) (11 (link)).
After three washes, embryos with normal develop-mental morphologies were randomly divided into 3 groups including:

α-MEM medium (Sigma, USA) containing 10% fetal bovine serum (FBS) and 10-9 M melatonin as the melatonin-treated group.

α-MEM medium with serum and without melatonin as the simple media group.

α-MEM medium containing serum and 10-9 M luzindole as the Luzindole-treated group.

Between 72–96 hr after initiation of embryo culture, the expanded blastocysts were randomly selected for further experiments and compared with the fourth group, in vivo developed blastocysts, as the control group.

Moshkdanian G., Moghani-Ghoroghi F., Pasbakhsh P., Nematollahi-Mahani S.N., Najafi A, & Kashani S.R. (2017). Melatonin upregulates ErbB1 and ErbB4, two primary implantation receptors, in pre-implantation mouse embryos. Iranian Journal of Basic Medical Sciences, 20(6), 655-661.

+ Open protocol

+ Expand

Rosette Leaf Trichome Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rosette leaves were detached from plants at 21DPI and placed between two microscope slides. The leaves were imaged using a Nikon D700 DSLR-CMOS sensor camera mounted on a Nikon SMZ-2T stereomicroscope. The “Volocity Demo” software was used to count the total number of hairs visible on each leaf surface, and ImageJ was used to calculate leaf surface areas for trichome density calculations.

Maxwell D.J., Partridge J.C., Roberts N.W., Boonham N, & Foster G.D. (2016). The Effects of Plant Virus Infection on Polarization Reflection from Leaves. PLoS ONE, 11(4), e0152836.

+ Open protocol

+ Expand

Tick Infestation on Passerine Birds in Hungary

Check if the same lab product or an alternative is used in the 5 most similar protocols

During a three year period (from January 2012 until December 2014) ixodid ticks were collected from passerine birds at three ringing stations in Hungary (Ócsa: 47.2967°, 19.2101°; Fenékpuszta: 46.7088°, 17.2427°; Bódva-völgy: 48.2934°, 20.7385°). Birds were captured by standard Ecotone mist-nets (Gdynia, Poland), 12 m in length, 2.5 m in height and with 16 mm mesh as described [7 ]. All captured birds were examined for the presence of ticks, which were removed with fine tweezers and put into 70 % ethanol (for storage) in separate vials according to their hosts. Morphological identification was done with a stereo microscope (SMZ-2 T, Nikon Instruments, Japan, illuminated with model 5000-1, Intralux, Switzerland) according to standard keys [13 , 14 (link)]. Characteristics (feeding site preference, migration distance and weight) were assigned to bird species based on ornithological observations [15 ].

Hornok S., Flaisz B., Takács N., Kontschán J., Csörgő T., Csipak Á., Jaksa B.R, & Kováts D. (2016). Bird ticks in Hungary reflect western, southern, eastern flyway connections and two genetic lineages of Ixodes frontalis and Haemaphysalis concinna. Parasites & Vectors, 9, 101.

+ Open protocol

+ Expand

Microscopic Analysis of Heated Starch

Check if the same lab product or an alternative is used in the 5 most similar protocols

Optical microscopy (Nikon, SMZ‐2 T, Japan) was used to observe the microstructure of the heated starch samples to identify any residual granule structures. Samples were diluted with buffer (1:10 wt/wt). A confocal laser scanning microscope (Model LSM 880, Carl Zeiss MicroImaging GmbH, Jena, Germany) was also used for microstructural characterization of some samples, after mixing with 0.5 wt% Rhodamine Blue (RB), excited at 514 nm, to fluorescently label the starch. Samples were excited with a He/Ne (543, 633 nm) laser source. A 20× objective with numerical aperture 0.5 was used to obtain all images, at 1,024 × 1,024 pixel resolution.

You K., Murray B.S, & Sarkar A. (2020). Rheology and tribology of starch + κ‐carrageenan mixtures. Journal of Texture Studies, 52(1), 16-24.

+ Open protocol

+ Expand

Morphological Analysis of Milla Complex

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plant morphology was analyzed from field collected and herbarium material (ARIZ, BH, CHAPA, F, FC, GH, IEB, JEPS, MEXU, NY, RSA, UAMIZ, US and XAL). We studied the floral morphology from organisms collected in the field, except for Millamortoniana (material removed from herbarium, MEXU). Morphological characters, vegetative and reproductive, were observed and analyzed with the help of a microscope (Nikon SMZ-2T) and terminology follows various authors (Moore 1953 , Lenz 1971 (link), Rahn 1998 (link), Harris and Harris 2001 ). In addition, floral and foliar anatomical characters (Gutiérrez et al. 2010 , 2015 (link)) were incorporated. The complete list of 60 characters and characters’ states used to delimit the monophyletic groups for the Milla complex can be consulted in Gutiérrez et al. (2017) (link). Those characters that were recovered as synapomorphies or unique character combinations are here used to construct the keys for the Milla complex genera and the species of the new genus here proposed.

Gutiérrez J, & Terrazas T. (2020). Xochiquetzallia (Asparagaceae, Brodiaeoideae), a new genus segregated from the paraphyletic Dandya. PhytoKeys, 139, 39-49.

+ Open protocol

+ Expand

Comparative Anatomical Study of Vomeronasal Organ

Check if the same lab product or an alternative is used in the 5 most similar protocols

For gross observations, seven heads (included the previous five CT heads) of the two species were fixed and preserved in a mixture of 10% formalin, 2% phenol, and 1% glycerin. These heads were dissected carefully to expose the brain, the vomeronasal nerves, the vomeronasal organ and its cartilage, and the nasopalatine duct in situ. Measurements were then made to the whole organ with a stainless Caliper. The average length and width of the VD were measured by the Image J program.
Cross sections of three frozen heads from both animal species were made for investigating the relation of the vomeronasal organ to the nasal concha and septum. The vomeronasal organ was crossed at its cranial, middle, and caudal parts, and then examined by using a stereomicroscope (Nikon SMZ-2T, Tokyo, Japan). The observations were recorded and photographed by using a digital camera (Sony DSC-W690, 16.1 MP). For the terminology, Nomina Anatomica Veterinaria [27 ] was used as if it is possible.

Mahdy E.A., El behery E.I, & Mohamed S.K. (2019). Comparative morpho-histological analysis on the vomeronasal organ and the accessory olfactory bulb in Balady dogs (Canis familiaris) and New Zealand rabbits (Oryctolagus cuniculus). Journal of Advanced Veterinary and Animal Research, 6(4), 506-515.

+ Open protocol

+ Expand

Isolation and culture of sheep preantral follicles

Check if the same lab product or an alternative is used in the 5 most similar protocols

The ovaries were cut into two halves and the medulla was removed. The ovarian cortices were cut into thin slices using a sterile surgical blade. These cortical slices were placed in the handling medium and intact preantral follicles (100–400 μm) in the size range of 250–400 μm were mechanically isolated by micro dissection under a stereo zoom microscope (SMZ 2T, Nikon corporation, Japan) using two 26 gage needles fitted to 1 mL syringe barrels. Care was taken to leave small amount of stromal tissue to avoid damage to the basement membrane. Preantral follicles having centrally placed, spherical oocytes with no signs of atresia and with intact basement membrane were considered good for culture (Fig 1a).Fig. 1

In vitro development of preantral follicles in sheep (A) Typical preantral follicle selected for culture (B) Preantral follicle cultured for 2 days. Note the early development of antrum (C) Preantral follicle cultured for 4 days. Note the large antrum pushing the COC to one side within the follicle (D) Preantral follicles cultured for 6 days. (E) COC collected from 6 day cultured Preantral follicles. (F) Oocyte from COC of 6 day cultured follicle matured in vitro for 24 h to MII stage as observed by Hoechst Staining.

Fig. 1:

Kona S.S., Kumar A.V., Punyakumari B., Kumar R.V, & Rao V.H. (2021). Influence of TCM 199B, α-MEM, Waymouth MB 752/1 culture media, VEGF, Estradiol-17β, GDF-9 and FGF on in vitro development of preantral follicles in sheep.. Veterinary and Animal Science, 13, 100189.

+ Open protocol

+ Expand

Shear Bond Strength Evaluation of Orthodontic Brackets

Check if the same lab product or an alternative is used in the 5 most similar protocols

The shear bond strength (SBS) test was performed in a universal testing machine (Pearson Panke Equipment Ltd., London), with a 50 kg load cell, at a speed of 1.0 mm/min until the removal of the brackets. The chisel was positioned parallel to the surface of the tooth/bracket interface, to allow force transmission in the occluso-gingival direction (Fig 3). The results obtained were converted to megapascals (MPa) by dividing the debonding force (in Newton) by the bracket base area (10.64 mm²). Immediately after bracket debonding, the enamel surface of each specimen was examined under 10× magnification with a stereoscopic microscope (Nikon, SMZ-2T, Japan), to determine the amount of residual adhesive. Adhesive remnant index (ARI) scores at the failure sites were recorded according to the classification of Artun and Bergland,¹⁶ as follows: score 0, no adhesive left on the tooth; score 1, less than half of the adhesive left on the tooth; score 2, more than half of the adhesive left on the tooth; and score 3, all of the adhesive left on the tooth.
Figure 3Universal testing machine used for determining the shear bond strength.

Alhasyimi A.A., Pudyani P.S, & Hafizi I. (2018). Effect of mangosteen peel extract as an antioxidant agent on the shear bond strength of orthodontic brackets bonded to bleached teeth. Dental Press Journal of Orthodontics, 23(5), 58-64.

+ Open protocol

+ Expand

Microscopic Analysis of Cellulose-BmimAc-Oil Mixtures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cellulose–BmimAc–oil
mixtures were analyzed using a light microscope (Nikon, SMZ-2T, Japan),
equipped with a digital camera (Leica MC120 HD) and 10×/20×
lenses. A drop of solution was placed on a welled slide and covered
with a coverslip (0.17 mm thickness). Images were processed using
the image analysis software ImageJ.

Lefroy K.S., Murray B.S, & Ries M.E. (2022). Effect of Oil on Cellulose Dissolution in the Ionic Liquid 1-Butyl-3-methyl Imidazolium Acetate. ACS Omega, 7(42), 37532-37545.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!