Icam 1

Manufactured by BioLegend

Sourced in United States

ICAM-1 is a cell surface glycoprotein that serves as an intercellular adhesion molecule. It is involved in the binding of leukocytes to endothelial cells during the inflammatory response.

Automatically generated - may contain errors

Lab products found in correlation

Vcam 1, by BioLegend (6 mentions) E selectin, by BioLegend (2 mentions) E selectin, by BD (2 mentions) Pecam1, by BioLegend (2 mentions) 4 laser confocal microscope, by Nikon (2 mentions) Cd146, by Miltenyi Biotec (1 mentions) Mhc class 1, by BioLegend (1 mentions) Mhc class 2, by Miltenyi Biotec (1 mentions) Accutase, by Innovative Cell Technologies (1 mentions) A11070, by Thermo Fisher Scientific (1 mentions) A11011, by Thermo Fisher Scientific (1 mentions) Fv1000, by Olympus (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Vcam 1, by Abcam (1 mentions) Ulex europaeus agglutinin 1, by Vector Laboratories (1 mentions) Blocking solution, by Merck Group (1 mentions) Lsm 700, by Zeiss (1 mentions) Anti cd68 antibody, by Abcam (1 mentions) Tissuefax, by TissueGnostics (1 mentions)

Spelling variants of this product

Anti-ICAM-1 (10 citations) Anti-ICAM-1-PE (5 citations) Anti‐ICAM‐1 antibody (3 citations) ICAM-1-PE-Cy7 (2 citations) Recombinant mouse ICAM-1-Fc (2 citations) FITC anti-human ICAM-1/CD54 (2 citations) ICAM-1/Fc chimera (2 citations) Rm) ICAM-1 (2 citations) ICAM-1-Fc (2 citations) ICAM-3 (clone CBR-IC3/1) (2 citations)

17 protocols using icam 1

Glomerular Endothelial Cell Phenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Accutase (Innovative Cell Technologies) was used to remove GEnC from the
plate using primarily EDTA chelation of Mg²+ and Ca²+ to
remove cells, resulting in minimal digestion of cell surface antigens. After
washing, 250,000 GEnC were aliquoted into cluster tubes and stained for CD146
(Miltenyi, Cat N: 130-102-230), ICAM1 (BioLegend, clone YN1/1.7.4), VCAM1
(BioLegend, clone 429 (MVcam.A), MHC class I (BioLegend, clone 28-8-6), MHC
class II (Miltenyi, Cat N: 130-102-139) or P-selectin (Miltenyi, Cat N:
130-105-538). Following staining, GEnC were washed, fixed with 2%
paraformaldehyde in PBS, and analyzed by flow cytometry (BD LSR Fortessa or
Accura). Flow analyses were performed in FlowJo version 10.

Wilson N.A., Dylewski J., Degner K.R., O’Neill M.A., Reese S.R., Hidalgo L.G., Blaine J, & Panzer S.E. (2019). An in vitro model of antibody-mediated injury to glomerular endothelial cells: upregulation of MHC class II and adhesion molecules. Transplant immunology, 58, 101261.

+ Open protocol

+ Expand

Fluorescent Labeling of Microvascular Networks

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mature microvascular networks were rinsed with warm PBS followed by the addition of approximately 100 μl of 4% paraformaldehyde (Electron Microscopy Sciences, # 15700) to the media channels and left at room temperature. After 15 min of fixation, devices were rinsed twice with PBS, and blocking solution (4% bovine serum albumin, 0.5% goat serum) (Sigma-Aldrich) was added. Devices were incubated for 1 day at 4°C, washed with PBS, and stained with primary antibodies: ICAM-1 (Biolegend, 4453320),VCAM-1 (Abcam, ab134047), CD31 (Abcam, ab28364), conjugated Alexa Fluor 647 anti-human CD326 (EPCAM) (BioLegend, 324212), Acti-stain 555 phalloidin, F-actin (Cytoskeleton, PHDH1-A) and incubated at 4°C for another day. Devices were again washed with PBS and secondary antibodies (Thermo Fisher Scientific, A-11070, A-11011, A-21052) DAPI (4′,6-Diamidino-2-Phenylindole, Dihydrochloride, Invitrogen) or DyLight 649 labeled Ulex Europaeus Agglutinin I (Vector Laboratories) were added, followed by incubation at 4°C protected from light. Finally, samples were washed again with PBS and 3D images were acquired with a confocal microscope (Olympus FV1000) at 20×. Z-stacks were collapsed with maximum intensity projections for viewing (800 × 800 pixels) using FIJI (22 (link)).

Campisi M., Sundararaman S.K., Shelton S.E., Knelson E.H., Mahadevan N.R., Yoshida R., Tani T., Ivanova E., Cañadas I., Osaki T., Lee S.W., Thai T., Han S., Piel B.P., Gilhooley S., Paweletz C.P., Chiono V., Kamm R.D., Kitajima S, & Barbie D.A. (2020). Tumor-Derived cGAMP Regulates Activation of the Vasculature. Frontiers in Immunology, 11, 2090.

+ Open protocol

+ Expand

Multiparametric Plaque Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

CD45-positive cells were stained using a suitable antibody (Biolegend). Macrophage content of the atherosclerotic lesion was analyzed using an anti-CD68 antibody (Abcam). Additionally anti-CD146, anti-CD4 (both Abcam), anti-CD8 (Bioss Antibodies), and MPO (R&D Systems) antibodies were used for the differentiation of cell populations within the plaque. ICAM-1 as well as E-selectin (both Biolegend) staining was performed to visualize adhesion molecules on the plaque surface/area. A Zeiss Axio observer microscope equipped with a Hamamatsu Orca Flash (Hamamatsu) was used for fluorescence microscopy, whereas a Zeiss LSM 700 (Carl Zeiss AG) was utilized for broad-field imaging. Tissue sections were automatically analyzed using TissueFAXS (TissueGnostics) and ImageJ (NIH). All analyses were carried out by a blinded member of the study team.

Lenz M., Salzmann M., Ciotu C.I., Kaun C., Krychtiuk K.A., Rehberger Likozar A., Sebestjen M., Goederle L., Rauscher S., Krivaja Z., Binder C.J., Huber K., Hengstenberg C., Podesser B.K., Fischer M.J., Wojta J., Hohensinner P.J, & Speidl W.S. (2022). Pharmacologic modulation of intracellular Na+ concentration with ranolazine impacts inflammatory response in humans and mice. Proceedings of the National Academy of Sciences of the United States of America, 119(29), e2207020119.

+ Open protocol

+ Expand

Simvastatin and Benznidazole Effects on E-selectin, ICAM-1, and VCAM-1 in Trypanosoma-Infected Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

5×10⁵ EA.hy926 or HUVEC cells/well were seeded in 6-well plates and exposed to various simvastatin and/or benznidazole concentrations for 24 hours. Then, cells were infected with trypomastigotes at a MOI of 10 and incubated for 16 hours. Cells were detached with 1X EDTA-PBS at 0.5 mM. The harvested cells were washed with cold PBS and centrifuged at 800 x g for 5 minutes. Then, cells were washed with flow cytometry buffer three times. 100 μL of cell suspensions were incubated for 45 min at 4°C in the dark with mouse monoclonal anti- human E-selectin (5 μL undiluted), ICAM-1(3 μL undiluted), and VCAM-1 (1 μL undiluted) that were conjugated with PE, FITC and APC, respectively (BioLegend, USA). Cell suspensions were analyzed by flow cytometry using a FACSAria-III flow cytometer (BD Biosciences, USA). A homogeneous cell population was selected by size vs. granularity in log scale for these two conditions.

Campos-Estrada C., Liempi A., González-Herrera F., Lapier M., Kemmerling U., Pesce B., Ferreira J., López-Muñoz R, & Maya J.D. (2015). Simvastatin and Benznidazole-Mediated Prevention of Trypanosoma cruzi-Induced Endothelial Activation: Role of 15-epi-lipoxin A4 in the Action of Simvastatin. PLoS Neglected Tropical Diseases, 9(5), e0003770.

+ Open protocol

+ Expand

Endothelial Cell Surface Marker Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

After cold storage, ECs were warm-reperfused with/without 1μM NIM811 for 6 or 24 hours. ECs were detached, washed, and resuspended in PBS supplemented with 1% BSA (Fisher Scientific, NH) and 10μg/mL DNAse I (Sigma-Aldrich, MO). ECs were then FcR-blocked at 10μL/mL (eBioscience, CA) and stained with fluorophore-conjugated antibodies against ICAM-1, VCAM-1, PD-L1, MHC-I (BioLegend, CA), E-selectin, CD80 (BD Biosciences, NJ), and CD86 (eBioscience, CA). Mean fluorescence intensity (MFI) was determined by Guava easyCyte 8HT flow cytometer (Merck Millipore, MA). Data were processed with FCS Express 4 (De Novo Software, CA).

Tran D.T., Esckilsen S., Mulligan J., Mehrotra S., Atkinson C, & Nadig S.N. (2018). Impact of Mitochondrial Permeability on Endothelial Cell Immunogenicity in Transplantation. Transplantation, 102(6), 935-944.

+ Open protocol

+ Expand

Immune Cell Receptor Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were preincubated with 10 μg/ml isotype controls or blocking antibodies against LFA-1 (clone: HI111, Biolegend), ICAM-1 (clone: HCD54, Biolegend), ICAM-2 (clone: CBR-IC2/2, eBioscience), ICAM-3 (clone: CBR-IC3/1, eBioscience), CD62 E/P-selectin (clone: BBIG-E6 (13D5), R&D) and IFN-I/IFNAR2 (39000-1, PBL) for 30 minutes, or with 3 μM single-stranded oligodeoxynucleotides ODN 20958 (TLR7 antagonist, Miltenyi) or ODN 2088 (TLR9 antagonist, Invivogen) for 5 hours, followed by stimulation in the presence of blocking reagents unless indicated otherwise.

Yun T.J., Igarashi S., Zhao H., Perez O.A., Pereira M.R., Zorn E., Shen Y., Goodrum F., Rahman A., Sims P.A., Farber D.L, & Reizis B. (2021). Human plasmacytoid dendritic cells mount a distinct antiviral response to virus-infected cells. Science immunology, 6(58), eabc7302.

+ Open protocol

+ Expand

Western Blotting and Immunostaining Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

For western blotting, antibodies were used as follows: TRF2 (1:500, Cell Signaling Technology, 13136), p16 (1:1000, Proteintech, 10883-1-AP), p21 Waf1/Cip1 (1:1000, Cell Signaling Technology, 2947), γ-H2AX (ser139) (1:500, Cell Signaling Technology, 9718), 53BP1 (1:1000, Novus, NB100-304), p53 (1:1000, Cell Signaling Technologies, 2524), P-p53 (1:1000, Cell Signaling Technologies, 9284), Myc (1:1000, Cell signaling, Technology 2276), P-TBK1 (Ser 172) (1:1000, Cell Signaling Technology, 5483), TBK1 (1:1000, Cell Signaling Technology, 3013), NF-κB P-p65 (Ser 536) (1:1000, Cell Signaling Technology, 3033), NF-κB p65 (1:1000, Cell Signaling Technology, 8242), NF-κB1 p105/p50 (1:1000, Cell Signaling Technology, 3035), cGAS (1:1000, Cell Signaling Technology, 15102), Actin (1:10 000, Sigma-Aldrich, A5-441), anti-rabbit HRP (1:3000, Cell Signaling Technology, 7074), anti-mouse HRP (1:3000, Cell Signaling Technology, 7076). For immunostaining: γ-H2AX (JBW301) (1:500, Merck, 05-636), H3K9me3 (1:400, Abcam, ab8898), 53BP1 (1:500, Novus, NB100-304), αSMA (Biotin) (1:500, Abcam, ab125057), CD45 (Alexa Fluor 647) (1:50, Biolegend, 103123), CD3 (Alexa Fluor 647) (1:50, Biolegend, 100209), CD68 (Alexa Fluor 647) (1:50, Biolegend, 137001), ICAM-1 (1:50, Biolegend, 116102), VCAM-1 (1:50, Biolegend, 105702).

Uryga A.K., Grootaert M.O., Garrido A.M., Oc S., Foote K., Chappell J., Finigan A., Rossiello F., d’Adda di Fagagna F., Aravani D., Jorgensen H.F, & Bennett M.R. (2021). Telomere damage promotes vascular smooth muscle cell senescence and immune cell recruitment after vessel injury. Communications Biology, 4, 611.

+ Open protocol

+ Expand

Quantifying Vascular Receptor Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

To investigate the relative expression of EC receptors in different organs, we intravenously injected 20 μg of antibodies against E-selectin, P-selectin (BD PharMingen), ICAM1, ICAM2, PECAM1, CD36 (BioLegend) and VCAM1 (Invitrogen) conjugated to FITC (VCAM1) or A647 (all other antibodies), into uninfected or day 6 infected mice, as previously described in the context of parasitology for CD31 (Hopp et al., 2015 ). We measured MFIs of at least 100 different vessels per organ in 3 separate mice using an LSM 710 Zeiss microscope, a 40x objective (1.3 NA).

De Niz M., Brás D., Ouarné M., Pedro M., Nascimento A.M., Henao Misikova L., Franco C.A, & Figueiredo L.M. (2021). Organotypic endothelial adhesion molecules are key for Trypanosoma brucei tropism and virulence. Cell Reports, 36(12), 109741.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Aortic Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

For cryosections, tissue was embedded in optimal cutting temperature, frozen on dry ice, and stored at −80°C. Aortic roots were sectioned on a cryostat to generate 10‐ to 15‐μm sections. Cryosections were fixed in acetone for 10 minutes at −20°C, blocked in IHC Tek antibody diluent (IHC World, Ellicott City, MD) for 1 hour at room temperature, and incubated with the indicated antibodies in IHC Tek antibody diluent buffer. Antibodies were phospho‐NFκB‐P65 (1:100; Cell Signaling Technology, Danvers, MA); FN (1:400; Sigma, St. Louis, MO); VCAM‐1 (1:200; BD Biosciences, San Jose, CA); ICAM‐1 (1:200; Biolegend, San Diego, CA); MMP9 (1:200; Abcam, Cambridge, UK); MMP2 (1:300; Millipore, Burlington, MA); CD45 (1:150; BD Pharmingen); CD68 (1:200; Abcam); SMA (1:200; Sigma); F480 (1:200; Serotec, Hercules, CA). Sections were washed 3 times in PBS and incubated with Alexa fluor 598‐conjugated donkey anti‐rabbit or ‐rat secondary antibody (Invitrogen, Carlsbad, CA) for 1 hour at room temperature. Slides were washed with PBS and mounted in Vectashield with DAPI (Vector Laboratories, Burlingame, CA). Images were acquired using a Nikon 4 laser confocal microscope. For histology, sections were stained with hematoxylin and eosin, Oil Red, or picrosirius red.

Budatha M., Zhang J., Zhuang Z.W., Yun S., Dahlman J.E., Anderson D.G, & Schwartz M.A. (2018). Inhibiting Integrin α5 Cytoplasmic Domain Signaling Reduces Atherosclerosis and Promotes Arteriogenesis. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 7(3), e007501.

+ Open protocol

+ Expand

Quantifying NF-κB and ICAM-1 Activation in MS1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

After stimulation of MS1 with S. mutans in antibiotic‐free DMEM at an MOI of 1, cells were lysed after the corresponding stimulation time, as described previously.²⁰ The total protein concentration was determined using a bicinchoninic acid (BCA) protein assay kit (Pierce Biotechnology). Western blot analysis was performed using antibodies specific for phospho‐NF‐κB p65 (Ser536) (93H1) (Cell Signaling, 3033S), NF‐κB p65 (D14E12) (Cell Signaling, 8242S), ICAM‐1 (BioLegend, 116101), and β‐actin (13E5) (Cell Signaling, 4970), along with the corresponding horseradish peroxidase‐conjugated secondary antibodies. The NF‐κB and ICAM‐1 levels were normalized to β‐actin by scanning densitometry using ImageJ software.

Yu L., Maishi N., Akahori E., Hasebe A., Takeda R., Matsuda A.Y., Hida Y., Nam J., Onodera Y., Kitagawa Y, & Hida K. (2022). The oral bacterium Streptococcus mutans promotes tumor metastasis by inducing vascular inflammation. Cancer Science, 113(11), 3980-3994.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!