T cho e test

Serum total cholesterol and triglyceride levels were measured using T-Cho E-test and TG E-test kits (Wako Pure Chemical, Osaka, Japan), respectively. The protocol given in the attached instruction manual was followed.

Dried feces (10 mg) were dissolved in 0.2 mL 90% ethanol and incubated at 65 °C for 1 h. The samples were then dissolved in 0.5 mL 90% ethanol. The total levels of bile acids in the plasma and feces were measured with the TBA-Test (Wako). Each liver tissue sample (0.1 g) was treated with Folch solution and homogenized. Total levels of cholesterol were measured by the T-cho E-Test (Wako). Extracted lipids were quantitatively analyzed. As a recovery standard, norcholic acid (Steraloids, Inc., Newport, RI; 1 mg/mL stock solution in 50% ethanol) was added to the ethanol. Filtrated extracts were centrifuged at 12,000 × g for 5 min and diluted 10 times in water. The diluted extracts were then analyzed using high-pressure liquid chromatography-tandem mass spectrometry (50 mm × 1 mm C8 column, buffered mobile phase) [32 (link)].