C3h heouj mice

Manufactured by Jackson ImmunoResearch

Sourced in Montenegro

C3H/HeOuJ mice are a strain of laboratory mice. They are a common model used in research studies.

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Lab products found in correlation

Female c3h hej mice, by Jackson ImmunoResearch (1 mentions) Tlr4lps d, by Jackson ImmunoResearch (1 mentions) Balb c, by Jackson ImmunoResearch (1 mentions) C57bl 6, by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

C3H/HeOuJ male mice Female C3H/HeOuJ mice C3H/HeOuJ C3H mice

6 protocols using c3h heouj mice

Murine Urinary Tract Infection Model

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All animal work was approved by The Institutional Animal Care and Use Committee of Children’s National Hospital under Animal Use Protocol #00030764. Procedures were performed in an ethical fashion. Prior to use, all animals were acclimated for 7 days after arrival to the animal facility. 24-week-old female C3H/HeOuJ mice (stock no: 000635, The Jackson Laboratory, Bar Harbor, ME) were used in this study.
Mice were anesthetized using 2% isoflurane. Any urine in the bladder was expressed by gently pressing on the lower abdomen. A 24g x ¾ inch angiocatheter (Clint Pharmaceuticals, Old Hickory, TN) was attached to a prepared 1 ml syringe containing the inoculant. The angiocath was lubricated (DynaLub Sterile Lubricating Jelly, Amazon, Seattle, WA) and transurethrally inserted into the bladder. 100 μL of the inoculant was instilled slowly into the bladder and the angiocatheter kept inserted for 30 seconds to prevent leakage of the inoculant.

Lee A.S., Lamanna O.K., Ishida K., Hill E., Nguyen A, & Hsieh M.H. (2022). A Novel Propidium Monoazide-Based PCR Assay Can Measure Viable Uropathogenic E. coli In Vitro and In Vivo. Frontiers in Cellular and Infection Microbiology, 12, 794323.

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Mouse Housing and Breeding Protocol

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Animal experiments were approved by UCSF Institutional Animal Care and Use Committee. Animals were housed in communal cages in a temperature- and humidity-controlled environment with 12 hour light/dark cycle and provided standard rodent chow and water ad libitum. Wild-type female CD1 mice were bred in the UCSF Laboratory Animal Resource Center. Female C3H/HeJ mice, which have a spontaneous mutation in TLR4 (Tlr4^lps-d) and their control background C3H/HeOuJ mice were purchased from Jackson Laboratories (Bar Harbor, Maine).

CIL O., PHUAN P.W., SON J.H., ZHU J.S., KU C.K., TABIB N.A., TEUTHORN A.P., FERRERA L., ZACHOS N.C., LIN R., GALIETTA L.J., DONOWITZ M., KURTH M.J, & VERKMAN A.S. (2016). Phenylquinoxalinone CFTR activator as potential prosecretory therapy for constipation. Translational research : the journal of laboratory and clinical medicine, 182, 14-26.e4.

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Pru p 3 Allergy Induction in Mice

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C3H/HeOuJ mice, which present normal TLR4, were obtained from Jackson Laboratories (Bar Harbor, ME) and used with the permission of the Institutional Animal Care and Use Committee (IACUC) (number LA11-00273). Six- to eight-week-old female mice were anesthetized, and abdominal fur was removed with depilatory cream (Veet, Reckitt Benckiser, Parsippany, NJ), immediately followed by exposure to Pru p 3 (100 ug), with or without lipid-ligand (10ug) in 50 ul of PBS spread on the abdominal skin to dry.
Mice were exposed weekly for a total of 6 exposures and challenged a week after the last exposure. Mice were challenged by intraperitoneal injection with increasing doses of Pru p 3. Body temperature was measured before and 30 minutes after challenge by rectal thermometer (WPI Instruments, Sarasota, FL).

Tordesillas L., Cubells-Baeza N., Gómez-Casado C., Berin C., Esteban V., Barcik W., O’Mahony L., Ramirez C., Pacios L.F., Garrido-Arandia M, & Díaz-Perales A. (2017). Mechanisms underlying induction of allergic sensitization by Pru p 3. Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 47(11), 1398-1408.

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High-Energy Diet Effects on Mice

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Adult male C3H/HeOuJ mice were purchased from The Jackson Laboratory (Bar Harbor, ME) and housed in the UCSF vivarium for a minimum of two weeks before starting experimental diets. The mean weight of the mice at the start of the diet study was 25.7 ± 0.3 g. In the study protocol, mice were fed either chow (PicoLab 5053, LabDiet, Inc., St. Louis, MO) or custom high-energy diets comprising 60% kcal carbohydrate, 20% kcal fat and 20% kcal protein (Dyets Inc., Bethlehem, PA). The detailed composition of the custom diets is shown in Table 1. Mice were maintained on experimental diets for 3 or 12 wk. Animals were weighed and food consumption measured twice weekly. Mice were fasted for 4 h prior to killing. Procedures for animal care and euthanasia followed the guidelines set by the American Veterinary Association, and all protocols were approved by the Committee on Animal Research at the University of California, San Francisco.

Pierce A.A., Duwaerts C.C., Soon R.K., Siao K., Grenert J.P., Fitch M., Hellerstein M.K., Beysen C., Turner S.M, & Maher J.J. (2015). Isocaloric manipulation of macronutrients within a high-carbohydrate/moderate-fat diet induces unique effects on hepatic lipogenesis, steatosis and liver injury. The Journal of nutritional biochemistry, 29, 12-20.

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C3H/HeOuJ Mice Animal Study

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All animal studies complied with NIH guidelines and were approved by the Animal Use and Care Committee of Colorado State University. Three-week-old female C3H/HeOuJ mice were purchased from the Jackson Laboratories (Bar Harbor, ME). Animals were maintained in a BSL-3 facility at Colorado State University and had ad libitum access to water and chow.

Dutt T.S., Karger B.R., Fox A., Youssef N., Dadhwal R., Ali M.Z., Patterson J., Creissen E., Rampacci E., Cooper S., Podell B.K., Gonzalez-Juarrero M., Obregon-Henao A, & Henao-Tamayo M. (2022). Mucosal exposure to non-tuberculous mycobacteria elicits B-cell mediated immunity against pulmonary tuberculosis. Cell reports, 41(11), 111783.

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Immune Modulation in Murine Melanoma

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6–8 weeks old female C57BL/6, Balb/c, and C3H/HeOUJ mice (Jackson Lab) were housed according to the Johns Hopkins Hospital (JHH) Animal Care Committee. C57BL/6 MyD88^−/−TRIF^−/−, and C57BL/6 (Cg) Rag2tml (Rag2^−/−) mice were obtained from Drs. Franck Housseau (JHH). B16 and B16 GM-vaccine cells were cultured in RPMI1640 media containing 10% ΔFCS, penicillin (100U/ml) and streptomycin (100U/ml). In PD-L1 experiments, B16 were cultured with serum free media. CD11c+ cells were isolated by anti-mouse CD11c microBeads (MACS, Miltenyi Biotec). CD4 depleting GK1.5 antibody and CD8 depleting 2.43 (Bio X Cell) at 200μg/dose were injected intraperitoneally (i.p.) every 2 days. Hybridoma expressing blocking anti-PD-1 antibody (clone G4) was obtained from Dr. Charles Drake (JHH).

Fu J., Malm I.J., Kadayakkara D.K., Levitsky H., Pardoll D, & Kim Y.J. (2014). Preclinical evidence that PD-1 blockade cooperates with cancer vaccine TEGVAX to elicit regression of established tumors. Cancer research, 74(15), 4042-4052.

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