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4 protocols using creatine phosphate disodium salt tetrahydrate

1

Biochemical Assay for Uric Acid

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ATP, AMP, creatine phosphate disodium salt tetrahydrate, and creatine phosphokinase type I from rabbit muscle were purchased from Sigma-Aldrich (St. Louis, MO, USA) and [8-14C]-uric acid (53 mCi/mmol) were purchased from American Radiolabeled Chemicals (St. Louis, MO, USA). All other chemicals used were commercially available and of analytical grade.
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2

Characterization of Uric Acid Transporters

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The following compounds were purchased commercially from the sources indicated: allopurinol, benzbromarone, cyclosporine, D-fructose, elacridar, furosemide, hydrochlorothiazide, nicotinic acid, oxypurinol, rosuvastatin calcium salt, salicylic acid, 4-hydroxy chalcone (Wako Fine Chemical, Osaka, Japan); atorvastatin, chlorothiazide, febuxostat, mizoribine, pyrazinecarboxylic acid, ribavirin, tacrolimus, xylitol (Tokyo Chemical Industry, Tokyo, Japan); ethambutol, losartan (LKT Laboratories, St Paul, MN, USA); fenofibrate, probenecid, sulfasalazine, Ko143, ATP, AMP, creatine phosphate disodium salt tetrahydrate, creatine phosphokinase type I from rabbit muscle (Sigma-Aldrich, St. Louis, MO, USA); pyrazinamide (ACROS ORGANICS, Geel, Belgium); theophylline (Nacalai Tesque, Kyoto, Japan); and topiroxostat (MedChem Express, Princeton, NJ, USA). The [8-14C]-uric acid (53 mCi/mmol) was from American Radiolabeled Chemicals (St. Louis, MO, USA). All other chemicals used were commercially available and of analytical grade.
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3

Enzymatic Assay for Uric Acid

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ATP, AMP, creatine phosphate disodium salt tetrahydrate, and creatine phosphokinase type I from rabbit muscle were purchased from Sigma-Aldrich (St. Louis, MO, USA), and [8-14C]-uric acid (55 mCi/mmol) was purchased from American Radiolabeled Chemicals (St. Louis, MO, USA). All other chemicals were commercially available and of analytical grade.
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4

Metabolite Quantification Assay

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Indoxyl sulfate potassium salt, ATP, adenosine monophosphate (AMP), creatine phosphate disodium salt tetrahydrate, and creatine phosphokinase type I from rabbit muscles were obtained from Sigma Aldrich (St. Louis, MO). d4-IS potassium salt was purchased from Toronto Research Chemicals (North York, Canada). Adenine, hippuric acid, and creatinine were obtained from Wako Pure Chemical Industries (Osaka, Japan). All other chemicals used were commercially available and were of reagent or analytical grade.
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