Ubiquitin ab7780

RNeasy kit, reverse transcription kit, and QuantiTect SYBR Green PCR kit were purchased from Qiagen Inc., Valencia, CA. qPCR primers were designed using Primer Bank (https://pga.mgh.harvard.edu/primerbank/) or Primer BLAST website and purchased from Integrated DNA Technologies, Coralville, IA. Trans-AM Nrf2 kit (50296) for determination of Nrf2-ARE binding activity was obtained from Active Motif, Carlsbad, CA. GCLC (ab41463), NQO1 (ab34173), GSR (ab16801), and Ubiquitin (ab7780) antibodies were procured from Abcam, Cambridge, MA; rabbit anti-GAPDH (D16H11) from Cell Signaling, USA. Anti-rabbit or mouse secondary antibodies for immunoblots (horse radish peroxidase conjugated with IgG) were purchased from Vector Laboratories, Burlingame, CA. Protein Assay reagent (#500–0006) was procured from Bio-Rad, Hercules, CA. All other chemicals including oxidized glutathione, RNAlater, meta-phosphoric acid, and isoproterenol, were purchased from Sigma-Aldrich unless otherwise stated.

BAX (50599-2-Ig), BCL-2(12789-1-AP), Caspase-3(19677-1-AP), PARP-1(13371-1-AP), Flag (66008-4-Ig and 20543-1-AP), GFP (66002-1-Ig and 50430-2-AP), and HA (66006-2-Ig and 51064-2-AP) antibodies were purchased from Proteintech. β-actin antibody (sc-1616) was purchased from Santa Cruz Biotechnology. TRIM65 antibody (ARP34737) was purchased from Aviva Systems Biology. TOX4 antibody (HPA017880) was purchased from Atlas Antibodies. Ubiquitin (ab7780), ubiquitin (K48, ab140601), and ubiquitin (K63, ab179434) antibodies were purchased from Abcam. SiRNAs targeting TOX4 were purchased from Santa Cruz Biotechnology. The protein synthesis inhibitor cycloheximide (CHX), Pan caspase inhibitor Z-VAD-FMK, and the protease inhibitor MG132 were purchased from Sigma-Aldrich.