Analytical standards for 17α‐ethinylestradiol (EE2), 17β‐estradiol (E2β), diethylstilbestrol (DES), estrone (E1), estriol (E3), hexestrol (HEX), progesterone (PS), testosterone (TS), 4‐androstene‐3,17‐dione (AD), 17α‐methyltestosterone (MTS), testosterone propionate (TSP), as well as internal standards (IS) for β‐estradiol‐d2 (E2‐d2) and progesterone‐d9 (PS‐d9), were all obtained from Sigma Aldrich. Additionally, 17α‐estradiol (E2α) and 17α‐hydroxyprogesterone (HPS) were purchased from Dr. Ehrenstorfer. Dienestrol (DE) and α‐zearalanol (ZAL) were supplied through TCI EUROPE N.V., and Kanto Chemical Co., Inc, respectively. IS for diethylstilbestrol‐d8 (DES‐d8) and hexestrol‐d4 (HEX‐d4) were procured from Toronto Research Chemicals Inc. Bondesil C18 bulk sorbent and Bondesil PSA bulk sorbent were acquired from Agilent. HPLC‐grade acetonitrile and ammonia solvents were purchased from J. T. Baker, and Merck Millipore, respectively. Lastly, Waters Xbridge BEH C18, used to analyze negatively charged hormones, was obtained from Waters while Phenomenex Gemini C18, used to analyze positively charged hormones, was obtained from Phenomenex.
Bondesil c18 bulk sorbent
Manufactured by Agilent Technologies
Sourced in United States
Bondesil C18 is a reversed-phase sorbent material manufactured by Agilent Technologies. It is composed of silica particles bonded with octadecyl (C18) functional groups. This sorbent is commonly used in solid-phase extraction (SPE) applications to selectively retain and concentrate analytes from complex sample matrices.
Automatically generated - may contain errors
Lab products found in correlation
Gemini c18, by Phenomenex (1 mentions)
Progesterone, by Merck Group (1 mentions)
17α ethinylestradiol, by Merck Group (1 mentions)
Testosterone propionate, by Merck Group (1 mentions)
Progesterone d9, by Merck Group (1 mentions)
Milli q purification system, by Merck Group (1 mentions)
Primary secondary amine, by Merck Group (1 mentions)
Anhydrous magnesium sulphate mgso4, by Merck Group (1 mentions)
Formic acid ch2o2, by Merck Group (1 mentions)
Ammonium formate hcoonh4, by Merck Group (1 mentions)
Glacial acetic acid ch3cooh, by Merck Group (1 mentions)
2 protocols using bondesil c18 bulk sorbent
1
Analytical Standards for Steroid Hormone Analysis
2
Sensitive Pesticide Analysis via LC-MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
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LC-MS grade acetonitrile (MeCN), methanol (MeOH) and formic acid
(CH2O2) were acquired from Sigma-Aldrich (Steinheim,
Germany). Glacial acetic acid (CH3COOH) and ammonium formate
(HCOONH4) were from Merck (Darmstadt, Germany). Ultrapure water
(18.2 MΩ) was obtained using a Milli Q purification system (Millipore,
Molsheim, France). Anhydrous magnesium sulphate (MgSO4)
(Sigma-Aldrich), anhydrous sodium acetate
(C2H3NaO2) (Merck) and primary-secondary
amine (PSA, 40 μm particle size) (Supelco, Bellefonte, PA, USA) were
analytical purity. Bondesil C18 bulk sorbent (50 μm particle size) was
from Agilent Technologies (Santa Clara, CA, USA).
Certified standards of 507 pesticides were from Dr. Ehrenstorfer (Augsburg,
Germany), ChemService (West Chester, PA, USA), Sigma-Aldrich (Steinheim,
Germany) and A2S Analytical Standard Solutions (Saint Jean d’Illac,
France). The purity of standards was ≥92%. Four stock solutions
for GC-amenable pesticides and five stock solutions for LC-amenable pesticides
were prepared at 10 mg/L in acetonitrile [or methanol/acetonitrile (50:50, v/v),
depending on the analytes’ solubility] to cover all target analytes.
These nine multi-standard mixture solutions were used for the preparation of
matrix-matched calibration standards (1–100 μg/kg for LC-amenable
and 2–250 μg/kg for LC-amenable analytes) and validation
study.
(CH2O2) were acquired from Sigma-Aldrich (Steinheim,
Germany). Glacial acetic acid (CH3COOH) and ammonium formate
(HCOONH4) were from Merck (Darmstadt, Germany). Ultrapure water
(18.2 MΩ) was obtained using a Milli Q purification system (Millipore,
Molsheim, France). Anhydrous magnesium sulphate (MgSO4)
(Sigma-Aldrich), anhydrous sodium acetate
(C2H3NaO2) (Merck) and primary-secondary
amine (PSA, 40 μm particle size) (Supelco, Bellefonte, PA, USA) were
analytical purity. Bondesil C18 bulk sorbent (50 μm particle size) was
from Agilent Technologies (Santa Clara, CA, USA).
Certified standards of 507 pesticides were from Dr. Ehrenstorfer (Augsburg,
Germany), ChemService (West Chester, PA, USA), Sigma-Aldrich (Steinheim,
Germany) and A2S Analytical Standard Solutions (Saint Jean d’Illac,
France). The purity of standards was ≥92%. Four stock solutions
for GC-amenable pesticides and five stock solutions for LC-amenable pesticides
were prepared at 10 mg/L in acetonitrile [or methanol/acetonitrile (50:50, v/v),
depending on the analytes’ solubility] to cover all target analytes.
These nine multi-standard mixture solutions were used for the preparation of
matrix-matched calibration standards (1–100 μg/kg for LC-amenable
and 2–250 μg/kg for LC-amenable analytes) and validation
study.
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