Glutamax 1

The human breast adenocarcinoma cell lines MCF-7 (HTB-22) and MDA-MB-231 (HTB-26) were purchased from the American Type Culture Collection (ATCC). MCF-7 cells stably transfected with the full-length MT1-MMP vector (MCF-7 MT1-MMP) and MCF-7 cells transfected with the empty vector (MCF-7 VEC) were obtained as previously described (Maquoi et al., 2012 (link)). MCF-7 and MDA-MB-231 cell lines were cultured in DMEM (4,5 g/l glucose) with Glutamax I (PAN-Biotech, p04-04500) supplemented with 10% fetal bovine serum (Dominique Dutscher, S1810-500) and 1% penicillin-streptomycin (Invitrogen, 15140). Cultures were maintained at 37°C in a humidified atmosphere containing 5% CO₂ (v/v). Cells were routinely passaged at preconfluency using 0.05% trypsin, 0.53 mM EDTA (Invitrogen, 25300) and screened for the absence of mycoplasma using PCR methods.

The human lung carcinoma cell lines A549 (CCL-185) and the bronchial transformed cell line BZR (CRL-9483) were purchased from the American Type Culture Collection (ATCC). Both cell lines were cultured in DMEM (4,5 g/l glucose) with Glutamax I (PAN-Biotech, p04-04500) supplemented with 10% fetal bovine serum (Dominique Dutscher, S1810-500) and 1% penicillin-streptomycin (Invitrogen, 15140). Cultures were maintained at 37°C in a humidified atmosphere containing 5% CO2 (v/v). Cells were routinely passaged at preconfluency using 0.05% trypsin, 0.53 mM EDTA (Invitrogen, 25300) and screened for the absence of mycoplasma using PCR methods.