Cbm 20a communication bus module

Nuclear magnetic resonance (NMR) tests were conducted by using a Bruker Avance 400 M NMR (Billerica, MA, USA) at 298 K. Gel permeation chromatography (GPC) was performed using CBM-20A communication bus module (Kyoto, Japan) equipped with a RID-10A refractive index detector and LC-20AT liquid chromatograph from Shimadzu. Samples were passed through three series SB-802.5HQ, SB-806HQ and SB-804HQ columns using 3/1 methanol/water solution eluent at a flow rate of 0.5 mL min⁻¹. The number-average molecular weight (M_n) and polydispersity index (PDI) data are reported relative to polystyrene standards. Turbidity characterizations were conducted with a Beijing TU-1901 (Beijing, China) double beam UV-vis spectrophotometer in 0.5 mg mL⁻¹ copolymer solutions at 500 nm wavelength, the transmittances were recorded after the temperature was held for 3 min. Rheological behavior of the hydrogels was characterized by a HAAKE MARS III (Waltham, WA, USA) rheometer, equipped with a cone–plate (C35/1Ti) geometries. Scanning electron microscopy (SEM) image was observed on a JSM-7500 (Kyoto, Japan) microscope to determine the morphology of the micro porous material with operating voltage at 10 kV. Mechanical tensile tests of the hydrogels were performed using a uniaxial mechanical testing device (Xi’an, China) at a stretch rate of 50 mm min⁻¹.

Sample analysis was performed using a Shimadzu HPLC system consisting of LC-20AT pump, SIL-20AC autoinjector, CBM-20A communication bus module, CTO-20A column oven, and SPD-M20A diode array detector (Shimadzu, Kyoto, Japan). The separation of cefdinir was performed on a reverse phase C18 column (250 mm × 4.6 mm, 5 μm; Shiseido, Tokyo, Japan). The mobile phase consisted of 1 M tetramethylammonium hydroxide solution, water, 0.1 M ethylenediaminetetraacetic acid, and methanol (1.40:92.40:0.04:6.16, v/v/v/v) (pH 5, adjusted with diluted phosphoric acid), at a flow rate of 1.0 mL/min by modifying method in United States Pharmacopoeia (USP) (2010) [23 ]. The column and autosampler tray were maintained at 40 °C and 4 °C, respectively. The analytical run time was 10 min. UV detection was monitored at 254 nm and injection volume of sample was 10 μL. Data acquisition and processing were carried out using the Shimadzu LC Solution software.

The IP-RP-HPLC system comprised a DGU-20A3 degasser, an LC-20AD pump, a SIL-20AC autosampler, a CTO-20AC column oven, an SPD-20A UV/VIS detector, and a CBM-20A Communication Bus Module (Shimadzu). Instrumental control and data analysis were performed using LC Solution (Shimadzu). The nucleotides were separated using a Gemini 5 μm NX-C18 110 Å LC Column (150 × 4.6 mm) (Phenomenex, 00F-4454-E0) with a Gemini NX C18 Security Guard Cartridge (4.0 × 3.0 mm) (Phenomenex, AJ0-8368). The isocratic mobile phase consisted of 20% acetonitrile and 80% 30 mM phosphate buffer (pH 7.0) containing 10 mM tetrabutylammonium bromide (TBA-B). Isocratic elution was performed at 40 °C with a flow rate of 0.8 ml/min.

Instrumental analysis of the separated bioactive compounds from the matrix samples was conducted by HPLC using a Shimadzu CBM-20A Communication Bus Module to interact with a photodiode array detector in an ultraviolet-visible (UV-visible) M20A spectrometer, in which a LiChrospher^® 100 RP-18 column was fitted. The Fourier transform-infrared spectroscopy (FT-IR) instrument used was a Shimadzu IRAFFINITY-1S model at the scanning wavenumbers 500-4000 cm⁻¹. The LC mass spectrometry (LC-MS) instrument was an Acella Triple Quadrupole Quantum Access Thermo with a Hypersil Gold 0.2 µM column of 10 cm in length. The nuclear magnetic resonance (NMR) instrument was a JEOL ECS-400, and analyses were performed using D₂-grade methanol. All chemical reagents for HPLC, LC-MS, and sample preparation were of chromatographic and analytical grade.

A prominence UFLC system from Shimadzu (Kyoto, Japan) equipped with a CBM-20A Communication Bus Module, SPD-M20A Photodiode Array Detector, LC-20AD Pump, SIL-20A Autosampler, and CTO-20A Column Oven was used. After a 10 μL injection, the analytes were separated through a Hector-M C18 column (4.6 × 250 mm, 5 μm) from RStech (Daejeon, Korea) using mobile phase (A) 0.1% formic acid in water and (B) 0.1% formic acid in acetonitrile at a flow rate of 0.3 mL/min. The elution started at 0% B for 20 min and then was increased to 100% B for 110 min. The eluents were then detected by the LCMS-8040 system (Shimadzu) with an ESI interface source in positive and negative mode at 3.5 and −3.5 kV interface voltage, respectively. The MS source conditions were as follows: nebulizing gas flow rate, 3 L/min; desolvation line temperature, 250 °C; drying gas flow, 15 L/min; and heat block temperature, 400 °C.

HPLC fingerprints were measured with a Shimadzu Prominence system equipped with LC-20AT quaternary gradient pump, a SPD-M20A diode array detector (DAD), CBM-20A communication bus module, CTO-20A thermostatic column compartment, SIL-20A autosampler and Shimadzu LC solution software (Version 1.21 SP1).
A 3114/3236 TAM air microcalorimeter (Thermometric AB, Sweden) was used to determine the metabolic heat-flow power-time curves of P. aeruginosa growing in the LB culture medium. As an isothermal heat conduction calorimeter operating in the microwatt range, the microcalorimeter has eight calorimetric channels to keep the temperature in the range of 20–80 °C. All channels were mounted together to form a single heat-sink block housed in a temperature-controlled air thermostat. Each calorimetric channel was constructed in twin configuration with one side for the sample and the other side for a static reference. For more details about the instrument, refer to ref. 27 (link).